ArticleBiophysical journal2004
Using single-particle tracking to study nuclear trafficking of viral genes.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 125 citations in OpenAlex.
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Stochastic intracellular replication dynamics shape population-level evolution in Influenza A Virus.Virus evolution · 2026Article
- Human MX1 orchestrates the cytoplasmic sequestration of neosynthesized influenza A virus vRNPs.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Targeting colorectal cancer at the level of nuclear pore complex.Journal of advanced research · 2025Review
- Measuring single-virus fusion kinetics using an assay for nucleic acid exposure.Biophysical journal · 2022Article
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Nuclear Import of Adeno-Associated Viruses Imaged by High-Speed Single-Molecule Microscopy.Viruses · 2021Review
- Article
- Estimation of general time-varying single particle tracking linear models using local likelihood.Control Conference (ECC) ... European. European Control Conference · 2020Article
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
- Microtubules in Influenza Virus Entry and Egress.Viruses · 2020Review
- The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes.The Journal of cell biology · 2020Article
- RNA Sequence Features Are at the Core of Influenza A Virus Genome Packaging.Journal of molecular biology · 2019Review
- Production of Quantum Dots-containing Influenza Virus Particles for Studying Viral Uncoating Processes.Bio-protocol · 2019Article
- In vivo tracking of orally-administered particles within the gastrointestinal tract of murine models using multispectral optoacoustic tomography.Photoacoustics · 2019Article
- Quantum dots crack the influenza uncoating puzzle.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Real-time dissection of dynamic uncoating of individual influenza viruses.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms.Advances in experimental medicine and biology · 2019Review
- The parts are greater than the whole: the role of semi-infectious particles in influenza A virus biology.Current opinion in virology · 2018Review
- Imaging, Tracking and Computational Analyses of Virus Entry and Egress with the Cytoskeleton.Viruses · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The question of how genetic materials are trafficked in and out of the cell nucleus is a problem of great importance not only for understanding viral infections but also for advancing gene-delivery technology. Here we demonstrate a physical technique that allows gene trafficking to be studied at the single-gene level by combining sensitive fluorescence microscopy with microinjection. As a model system, we investigate the nuclear import of influenza genes, in the form of ribonucleoproteins (vRNPs), by imaging single vRNPs in living cells in real time. Our single-particle trajectories show that vRNPs are transported to the nuclear envelope by diffusion. We have observed heterogeneous interactions between the vRNPs and nuclear pore complexes with dissociation rate constants spanning two orders of magnitude. Our single-particle tracking experiments also provided new insights into the regulation mechanisms for the nuclear import of vRNPs: the influenza M1 protein, a regulatory protein for the import process, downregulates the nuclear import of vRNPs by inhibiting the interactions between vRNPs and nuclear pore complexes but has no significant effect on the transport properties of vRNPs. We expect this single-particle tracking approach to find broad application in investigations of genetic trafficking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.