ArticleBiophysical journal2004
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers.Photosynthesis research · 2025Article
- ApcE plays an important role in light-induced excitation energy dissipation in the Synechocystis PCC6803 phycobilisomes.Photosynthesis research · 2024Article
- Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome.Nature communications · 2022Article
- Observation of robust energy transfer in the photosynthetic protein allophycocyanin using single-molecule pump-probe spectroscopy.Nature chemistry · 2022Article
- Estimation of the relative contributions to the electronic energy transfer rates based on Förster theory: The case of C-phycocyanin chromophores.Biophysics and physicobiology · 2021Article
- Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.International journal of molecular sciences · 2017Review
- Dissecting pigment architecture of individual photosynthetic antenna complexes in solution.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- The specificity of controlled protein disorder in the photoprotection of plants.Biophysical journal · 2013Article
- Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap.Accounts of chemical research · 2012Article
- Single-molecule spectroscopy: caught in a trap.Nature chemistry · 2010Article
- Watching conformational- and photo-dynamics of single fluorescent proteins in solution.Nature chemistry · 2010Article
- Article
- Single-molecule spectroscopic study of enhanced intrinsic phycoerythrin fluorescence on silver nanostructured surfaces.Analytical chemistry · 2008Article
- Metal-Enhanced Fluorescence of Phycobiliproteins from Heterogeneous Plasmonic Nanostructures.The journal of physical chemistry. C, Nanomaterials and interfaces · 2007Article
- Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence images and time-resolved studies using single-molecule spectroscopy.Analytical chemistry · 2006Article
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5 authors.
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Abstract
We report on single-molecule fluorescence measurements performed on the phycobiliprotein allophycocyanin (APC). Our data support the presence of a unidirectional Förster-type energy transfer process involving spectrally different chromophores, alpha84 (donor) and beta84 (acceptor), as well as of energy hopping amongst beta84 chromophores. Single-molecule fluorescence spectra recorded from individual immobilized APC proteins indicate the presence of a red-emitting chromophore with emission peaking at 660 nm, which we connect with beta84, and a species with the emission peak blue shifted at 630 nm, which we attribute to alpha84. Polarization data from single APC trimers point to the presence of three consecutive red emitters, suggesting energy hopping amongst beta84 chromophores. Based on the single-molecule fluorescence spectra and assuming that emission at the ensemble level in solution comes mainly from the acceptor chromophore, we were able to resolve the individual absorption and emission spectra of the alpha84 and beta84 chromophores in APC.
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