ArticleBiophysical journal2004
Time-resolved detection of sensory rhodopsin II-transducer interaction.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography.Nature communications · 2025Article
- Light-induced reversible reorganizations in closed Type II reaction centre complexes: physiological roles and physical mechanisms.Open biology · 2022Review
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Transient conformational fluctuation of TePixD during a reaction.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.Chemical reviews · 2014Review
- Chimeric microbial rhodopsins containing the third cytoplasmic loop of bovine rhodopsin.Biophysical journal · 2011Article
- Protein-protein interaction changes in an archaeal light-signal transduction.Journal of biomedicine & biotechnology · 2010Article
- Reaction dynamics of halorhodopsin studied by time-resolved diffusion.Biophysical journal · 2009Article
- Charge stabilization in reaction center protein investigated by optical heterodyne detected transient grating spectroscopy.European biophysics journal : EBJ · 2008Article
- Photoreverse reaction dynamics of octopus rhodopsin.Biophysical journal · 2007Article
- Laser-induced transient grating analysis of dynamics of interaction between sensory rhodopsin II D75N and the HtrII transducer.Biophysical journal · 2007Article
- Tetramer formation kinetics in the signaling state of AppA monitored by time-resolved diffusion.Biophysical journal · 2006Article
- The lifetimes of Pharaonis phoborhodopsin signaling states depend on the rates of proton transfers--effects of hydrostatic pressure and stopped flow experiments.Photochemistry and photobiologyArticle
- Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dynamics of protein conformational change of Natronobacterium pharaonis sensory rhodopsin II (NpSRII) and of NpSRII fused to cognate transducer (NpHtrII) truncated at 159 amino acid sequence from the N-terminus (NpSRII-DeltaNpHtrII) are investigated in solution phase at room temperature by the laser flash photolysis and the transient grating methods in real time. The diffusion coefficients of both species indicate that the NpSRII-DeltaNpHtrII exists in the dimeric form in 0.6% dodecyl-beta-maltopyranoside (DM) solution. Rate constants of the reaction processes in the photocycles determined by the transient absorption and grating methods agree quite well. Significant differences were found in the volume change and the molecular energy between NpSRII and NpSRII-DeltaNpHtrII samples. The enthalpy of the second intermediate (L) of NpSRII-DeltaNpHtrII is more stabilized compared with that of NpSRII. This stabilization indicates the influence of the transducer to the NpSRII structure in the early intermediate species by the complex formation. Relatively large molecular volume expansion and contraction were observed in the last two steps for NpSRII. Additional volume expansion and contraction were induced by the presence of DeltaNpHtrII. This volume change, which should reflect the conformational change induced by the transducer protein, suggested that this is the signal transduction process of the NpSRII-DeltaNpHtrII.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.