ArticleBiophysical journal2004
Twisting and untwisting a single DNA molecule covered by RecA protein.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- SWI5-SFR1 reduces RAD51 recombinase extending units during filament assembly.Nucleic acids research · 2025Article
- Single-molecule insight into stalled replication fork rescue in Escherichia coli.Nucleic acids research · 2021Review
- Mismatch sensing by nucleofilament deciphers mechanism of RecA-mediated homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The many lives of type IA topoisomerases.The Journal of biological chemistry · 2020Review
- Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization.Methods in molecular biology (Clifton, N.J.) · 2018Article
- The dynamic interplay between DNA topoisomerases and DNA topology.Biophysical reviews · 2016Review
- The Dynamic Interplay Between DNA Topoisomerases and DNA Topology.Biophysical reviews · 2016Article
- Dynamics and Regulation of RecA Polymerization and De-Polymerization on Double-Stranded DNA.PloS one · 2013Article
- Probing Rad51-DNA interactions by changing DNA twist.Nucleic acids research · 2012Article
- Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments.Nature methods · 2010Article
- Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.Trends in cell biology · 2010Review
- Visualizing protein-DNA interactions at the single-molecule level.Current opinion in chemical biology · 2010Review
- Direct observation of twisting steps during Rad51 polymerization on DNA.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Single molecule studies of homologous recombination.Molecular bioSystems · 2008Review
- Expedient placement of two fluorescent dyes for investigating dynamic DNA protein interactions in real time.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
- Real-time measurements of the nucleation, growth and dissociation of single Rad51-DNA nucleoprotein filaments.Nucleic acids research · 2007Article
- Mechanism of RecA-mediated homologous recombination revisited by single molecule nanomanipulation.The EMBO journal · 2006Article
- Numerical investigation of sequence dependence in homologous recognition: evidence for homology traps.Biophysical journal · 2005Article
- Torque-limited RecA polymerization on dsDNA.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We study dsDNA-RecA interactions by exerting forces in the pN range on single DNA molecules while the interstrand topological state is controlled owing to a magnetic tweezers setup. We show that unwinding a duplex DNA molecule induces RecA polymerization even at moderate force. Once initial polymerization has nucleated, the extent of RecA coverage still depends on the degree of supercoiling: exerting a positive or negative torsional constraint on the fiber forces partial depolymerization, with a strikingly greater stability when ATPgammaS is used as a cofactor instead of ATP. This nucleofilament's sensitivity to topology might be a way for the bacterial cell to limit consumption of precious RecA monomers when DNA damage is addressed through homologous recombination repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.