ArticleBiophysical journal2004
Exchange of gating properties between rat cx46 and chicken cx45.6.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Calcium induced N-terminal gating and pore collapse in connexin-46/50 gap junctions.bioRxiv : the preprint server for biology · 2025Article
- Connexin channels and hemichannels are modulated differently by charge reversal at residues forming the intracellular pocket.Biological research · 2024Article
- Connexin Gap Junction Channels and Hemichannels: Insights from High-Resolution Structures.Biology · 2024Review
- Molecular mechanisms underlying enhanced hemichannel function of a cataract-associated Cx50 mutant.Biophysical journal · 2021Article
- Cell Systems Bioelectricity: How Different Intercellular Gap Junctions Could Regionalize a Multicellular Aggregate.Cancers · 2021Article
- Connexin 46 and connexin 50 gap junction channel properties are shaped by structural and dynamic features of their N-terminal domains.The Journal of physiology · 2021Article
- Specificity of the connexin W3/4 locus for functional gap junction formation.Channels (Austin, Tex.) · 2016Article
- Determinants of Cx43 Channel Gating and Permeation: The Amino Terminus.Biophysical journal · 2016Article
- The connexin46 mutant, Cx46T19M, causes loss of gap junction function and alters hemi-channel gating.The Journal of membrane biology · 2015Article
- Motifs in the permeation pathway of connexin channels mediate voltage and Ca (2+) sensing.Frontiers in physiology · 2014Review
- Cataract-associated D3Y mutation of human connexin46 (hCx46) increases the dye coupling of gap junction channels and suppresses the voltage sensitivity of hemichannels.Journal of bioenergetics and biomembranes · 2012Article
- Voltage-dependent conformational changes in connexin channels.Biochimica et biophysica acta · 2012Review
- Structural organization of intercellular channels II. Amino terminal domain of the connexins: sequence, functional roles, and structure.Biochimica et biophysica acta · 2012Review
- The N-terminal half of the connexin protein contains the core elements of the pore and voltage gates.The Journal of membrane biology · 2012Article
- Aspartic acid residue D3 critically determines Cx50 gap junction channel transjunctional voltage-dependent gating and unitary conductance.Biophysical journal · 2012Article
- Structure of the gap junction channel and its implications for its biological functions.Cellular and molecular life sciences : CMLS · 2011Review
- The role of amino terminus of mouse Cx50 in determining transjunctional voltage-dependent gating and unitary conductance.Biophysical journal · 2010Article
- Regulation of lens gap junctions by Transforming Growth Factor beta.Molecular biology of the cell · 2010Article
- A mutant connexin50 with enhanced hemichannel function leads to cell death.Investigative ophthalmology & visual science · 2009Article
- The GJA8 allele encoding CX50I247M is a rare polymorphism, not a cataract-causing mutation.Molecular vision · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cx46 and Cx50 are coexpressed in lens fiber cells where they form fiber-fiber gap junctions. Recent studies have shown that both proteins play a critical role in maintaining lens transparency. Although both Cx46 and Cx50 (or its chicken ortholog, Cx45.6) show a high degree of sequence homology, they exhibit marked differences in gap junctional channel gating, unitary gap junctional channel conductance, and hemichannel gating. To better understand which regions of the protein are responsible for these functional differences, we have constructed a series of chimeric Cx46-Cx45.6 gap junctional proteins in which a single transmembrane or intracellular domain of Cx45.6 was replaced with the corresponding domain of Cx46, expressed them in Xenopus oocyte pairs or N2A cells, and examined the resulting gap junctional conductances. Our results showed that four out of six of the chimeras induced high levels of gap junctional coupling. Of these chimeras, only Cx45.6-46NT showed significant changes in voltage-dependent gating properties. Exchanging the N-terminus had multiple effects. It slowed the inactivation kinetics of the macroscopic junctional currents so that they resembled those of Cx46, reduced the voltage sensitivity of the steady-state junctional conductance, and decreased the conductance of single gap junctional channels. Additional point mutations identified a uniquely occurring arginine in the N-terminus of Cx46 as the main determinant for the change in voltage-dependent gating.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.