ArticlePflugers Archiv : European journal of physiology2004
Concurrent measurements of the free cytosolic concentrations of H+ and Na+ ions with fluorescent indicators.
Article in Pflugers Archiv : European journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Spatiotemporal Dynamics of Lateral NaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Spatio-temporal dynamics of lateral NabioRxiv : the preprint server for biology · 2025Article
- Non-invasive Optical Biomarkers Distinguish and Track the Metabolic Status of Single Hematopoietic Stem Cells.iScience · 2020Article
- Elevated intracellular NaJournal of neurochemistry · 2017Article
- Extrusion versus diffusion: mechanisms for recovery from sodium loads in mouse CA1 pyramidal neurons.The Journal of physiology · 2016Article
- Pyramidal cells accumulate chloride at seizure onset.Neurobiology of disease · 2012Article
- AF17 facilitates Dot1a nuclear export and upregulates ENaC-mediated Na+ transport in renal collecting duct cells.PloS one · 2011Article
- Dot1a contains three nuclear localization signals and regulates the epithelial Na+ channel (ENaC) at multiple levels.American journal of physiology. Renal physiology · 2010Article
- Synaptically induced sodium signals in hippocampal astrocytes in situ.The Journal of physiology · 2009Article
- Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification.Experimental cell research · 2008Article
- Broad-spectrum effects of 4-aminopyridine to modulate amyloid beta1-42-induced cell signaling and functional responses in human microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- The weak bases NH(3) and trimethylamine inhibit the medium and slow afterhyperpolarizations in rat CA1 pyramidal neurons.Pflugers Archiv : European journal of physiology · 2005Article
- Sodium influx pathways during and after anoxia in rat hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a method for the concurrent measurement of intracellular [Na+] ([Na+ ]i) and pH (pHi) in cells co-loaded with SBFI, a Na+-sensitive fluorophore, and either carboxy SNARF-1 or SNARF-5F, H+-sensitive fluorophores. With the optical filters specified, fluorescence emissions from SBFI and either SNARF derivative were sufficiently distinct to allow the accurate measurement of [Na+]i and pHi in rat hippocampal neurons. Neither the Na+ sensitivity of SBFI nor the pH sensitivities of carboxy SNARF-1 or SNARF-5F was affected by the presence of a SNARF derivative or SBFI, respectively. In addition, the calibration parameters obtained in neurons single-loaded with SBFI, carboxy SNARF-1 or SNARF-5F were not significantly influenced by the presence of a second fluorophore. In contrast to the established weak sensitivity of SBFI for protons, both SNARF derivatives appeared essentially insensitive to changes in [Na+]i. The utility of the technique was demonstrated in neurons co-loaded with SBFI and SNARF-5F, which was found to have a lower p Ka in situ than carboxy SNARF-1. There were no significant differences in the changes in [Na+]i and pHi observed in response either to intracellular acid loads imposed by the NH4+ prepulse technique or to transient periods of anoxia in neurons single-loaded with SBFI or SNARF-5F or co-loaded with both probes. The findings support the feasibility of using SBFI in conjunction with either carboxy SNARF-1 or SNARF-5F to concurrently and accurately measure [Na+]i and pHi.
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