ArticlePflugers Archiv : European journal of physiology2004
Protein and gene expression of Ca2+ channel isoforms in murine colon: effect of inflammation.
Article in Pflugers Archiv : European journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Chemotherapy induced gastrointestinal toxicities.Advances in cancer research · 2022Review
- Interaction between hydrogen sulfide-induced sulfhydration and tyrosine nitration in the KATP channel complex.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Postranslational Modification of Ion Channels in Colonic Inflammation.Current neuropharmacology · 2015Review
- Voltage dependent potassium channel remodeling in murine intestinal smooth muscle hypertrophy induced by partial obstruction.PloS one · 2014Article
- Changes in the expression of smooth muscle contractile proteins in TNBS- and DSS-induced colitis in mice.Inflammation · 2013Article
- Mechanisms of motility change on trinitrobenzenesulfonic Acid-induced colonic inflammation in mice.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2012Article
- The role of β-arrestin2 in the mechanism of morphine tolerance in the mouse and guinea pig gastrointestinal tract.The Journal of pharmacology and experimental therapeutics · 2012Article
- Ion channel remodeling in gastrointestinal inflammation.Neurogastroenterology and motility · 2010Review
- Colonic inflammation alters Src kinase-dependent gating properties of single Ca2+ channels via tyrosine nitration.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- The effect of tyrosine nitration of L-type Ca2+ channels on excitation-transcription coupling in colonic inflammation.British journal of pharmacology · 2010Article
- Downregulation of CPI-17 contributes to dysfunctional motility in chronic intestinal inflammation model mice and ulcerative colitis patients.Journal of gastroenterology · 2008Article
- Intestinal dysmotility in inflammatory bowel disease: mechanisms of the reduced activity of smooth muscle contraction.Inflammopharmacology · 2005Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
L-Type voltage-dependent Ca2+ channels (L-VDCC) mediate calcium influx in response to membrane depolarization and regulate intracellular processes such as contraction, secretion, neurotransmission, and gene expression. Colonic inflammation significantly attenuates calcium currents in smooth muscle; however, the basis for this remains unclear. In this study we examined the protein and mRNA expression of two isoforms of Ca(v)1.2, encoded by either exon la or 1b. Both isoforms were detected by Western blots, immunohistochemistry and RT-PCR in smooth muscle cells. Neither the protein nor mRNA expression measured by real-time PCR of either isoforms was affected in colonic myocytes from dextran sulfate sodium-treated mice. In whole-cell voltage-clamp experiments, the amplitude of the calcium currents were decreased by almost 70% by inflammation. The calcium channel currents were attenuated by 50 +/- 3% by the c-src kinase specific inhibitor, PP2, in control cells but only 19 +/- 7% in cells from inflamed mice. These studies suggest that decreased calcium channel currents following colonic inflammation are not due to decreased expression but may result from altered regulation by the non-receptor cellular tyrosine kinase, c-src kinase.
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Registered trials
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