ArticlePflugers Archiv : European journal of physiology2004
Targeted in vivo expression of proteins in the calyx of Held.
Article in Pflugers Archiv : European journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Super-Resolved Protein Imaging Using Bifunctional Light-Up Aptamers.Angewandte Chemie (International ed. in English) · 2024Article
- Maintenance of a central high frequency synapse in the absence of synaptic activity.Frontiers in cellular neuroscience · 2024Article
- Perceptual learning deficits mediated by somatostatin releasing inhibitory interneurons of olfactory bulb in an early life stress mouse model.Molecular psychiatry · 2023Article
- CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.The Journal of comparative neurology · 2021Article
- Article
- Structure-function relation of the developing calyx of Held synapse in vivo.The Journal of physiology · 2020Article
- Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem.Frontiers in neural circuits · 2019Article
- Article
- Pool size estimations for dense-core vesicles in mammalian CNS neurons.The EMBO journal · 2018Article
- Optogenetic manipulation of medullary neurons in the locust optic lobe.Journal of neurophysiology · 2018Article
- Secretory vesicle trafficking in awake and anaesthetized mice: differential speeds in axons versus synapses.The Journal of physiology · 2018Article
- The presynaptic scaffolding protein Piccolo organizes the readily releasable pool at the calyx of Held.The Journal of physiology · 2018Article
- A novel region in the CaeLife · 2017Article
- Ultrastructural and Functional Properties of a Giant Synapse Driving the Piriform Cortex to Mediodorsal Thalamus Projection.Frontiers in synaptic neuroscience · 2017Article
- In vivo matching of postsynaptic excitability with spontaneous synaptic inputs during formation of the rat calyx of Held synapse.The Journal of physiology · 2017Article
- Cortical Dependence of Whisker Responses in Posterior Medial Thalamus In Vivo.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
- Corticothalamic Spike Transfer via the L5B-POm Pathway in vivo.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
- Reconstitution of Giant Mammalian Synapses in Culture for Molecular Functional and Imaging Studies.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Ionotropic glutamate receptor GluA4 and T-type calcium channel Cav 3.1 subunits control key aspects of synaptic transmission at the mouse L5B-POm giant synapse.The European journal of neuroscience · 2015Article
- Convergence of cortical and sensory driver inputs on single thalamocortical cells.Cerebral cortex (New York, N.Y. : 1991) · 2014Article
Corrections and comments
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The calyx of Held serves as a model for synaptic transmission in the mammalian central nervous system. While offering unique access to the biophysics of presynaptic function, studies addressing the molecular mechanisms of neurotransmitter exocytosis in this model have been mainly limited to pharmacological interventions. To overcome this experimental limitation we used stereotaxic delivery of viral gene shuttles to rapidly and selectively manipulate protein composition in the calyx terminal in vivo. Sindbis or Semliki Forest viruses encoding enhanced green fluorescent protein (EGFP) were injected into the ventral cochlear nucleus (VCN) of rats (postnatal days 7-21) and yielded bright fluorescence in cells of the VCN, including globular bushy cells with their axon and calyx terminal. Fluorescence imaging and three dimensional reconstructions visualized developmental changes in calyx morphology. Small cytoplasmic and synaptic vesicle proteins were successfully overexpressed in the calyx. We extended two-photon microscopy to obtain simultaneous fluorescence and infrared scanning gradient contrast images, allowing for efficient patch-clamp recordings from EGFP-labelled calyces in acute brain slices (postnatal days 9-14). Recordings of spontaneous miniature excitatory postsynaptic currents and short-term depression in synapses overexpressing EGFP or synaptophysin-EGFP revealed normal synaptic function. Thus, Sindbis and Semliki Forest virus-directed overexpression of proteins in the calyx of Held provides a new avenue for molecular structure-function studies of mammalian central synapses.
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