ArticleJournal of virology2004
mRNA molecules containing murine leukemia virus packaging signals are encapsidated as dimers.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostability.Journal of virology · 2024Article
- Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus.Journal of virology · 2020Article
- Ribosome profiling of the retrovirus murine leukemia virus.Retrovirology · 2018Article
- The bifurcated stem loop 4 (SL4) is crucial for efficient packaging of mouse mammary tumor virus (MMTV) genomic RNA.RNA biology · 2018Article
- HIV-1 Sequence Necessary and Sufficient to Package Non-viral RNAs into HIV-1 Particles.Journal of molecular biology · 2017Article
- Review
- On the Selective Packaging of Genomic RNA by HIV-1.Viruses · 2016Review
- An immature retroviral RNA genome resembles a kinetically trapped intermediate state.Journal of virology · 2014Article
- Life of psi: how full-length HIV-1 RNAs become packaged genomes in the viral particles.Virology · 2014Review
- Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.Journal of virology · 2013Article
- Influence of untranslated regions on retroviral mRNA transfer and expression.BMC biotechnology · 2013Article
- A guanosine-centric mechanism for RNA chaperone function.Science (New York, N.Y.) · 2013Article
- Biosafety features of lentiviral vectors.Human gene therapy · 2013Review
- Diverse interactions of retroviral Gag proteins with RNAs.Trends in biochemical sciences · 2011Review
- The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles.Retrovirology · 2011Article
- Structural dynamics of retroviral genome and the packaging.Frontiers in microbiology · 2011Article
- Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography.Journal of molecular biology · 2010Article
- Definition of a high-affinity Gag recognition structure mediating packaging of a retroviral RNA genome.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Retroviral RNA dimerization and packaging: the what, how, when, where, and why.PLoS pathogens · 2010Review
- Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes.Retrovirology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prior work by others has shown that insertion of psi (i.e., leader) sequences from the Moloney murine leukemia virus (MLV) genome into the 3' untranslated region of a nonviral mRNA leads to the specific encapsidation of this RNA in MLV particles. We now report that these RNAs are, like genomic RNAs, encapsidated as dimers. These dimers have the same thermostability as MLV genomic RNA dimers; like them, these dimers are more stable if isolated from mature virions than from immature virions. We characterized encapsidated mRNAs containing deletions or truncations of MLV psi or with psi sequences from MLV-related acute transforming viruses. The results indicate that the dimeric linkage in genomic RNA can be completely attributed to the psi region of the genome. While this conclusion agrees with earlier electron microscopic studies on mature MLV dimers, it is the first evidence as to the site of the linkage in immature dimers for any retrovirus. Since the Psi(+) mRNA is not encapsidated as well as genomic RNA, it is only present in a minority of virions. The fact that it is nevertheless dimeric argues strongly that two of these molecules are packaged into particles together. We also found that the kissing loop is unnecessary for this coencapsidation or for the stability of mature dimers but makes a major contribution to the stability of immature dimers. Our results are consistent with the hypothesis that the packaging signal involves a dimeric structure in which the RNAs are joined by intermolecular interactions between GACG loops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.