ArticleNucleic acids research2004
Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- High matrix stiffness promotes senescence of type II alveolar epithelial cells by lysosomal degradation of lamin A/C in pulmonary fibrosis.Respiratory research · 2025Article
- Mitigating off-target effects in CRISPR/Cas9-mediated in vivo gene editing.Journal of molecular medicine (Berlin, Germany) · 2020Review
- Defining a standard and weighted mathematical index for maturation of dendritic cells.Immunology · 2018Article
- A novel conditional gene silencing method using a tumor-specific and heat-inducible siRNA system.Journal of industrial microbiology & biotechnology · 2016Article
- (Pro)renin receptor contributes to regulation of renal epithelial sodium channel.Journal of hypertension · 2016Article
- A novel placental like alkaline phosphatase promoter driven transcriptional silencing combined with single chain variable fragment antibody based virosomal delivery for neoplastic cell targeting [corrected].Journal of translational medicine · 2015Article
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- A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency.Cell & bioscience · 2015Article
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- Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction.American journal of physiology. Heart and circulatory physiology · 2012Article
- Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.Nucleic acids research · 2011Article
- Characterization of the cell of origin for small cell lung cancer.Cell cycle (Georgetown, Tex.) · 2011Article
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- Construction of siRNA/miRNA expression vectors based on a one-step PCR process.BMC biotechnology · 2009Article
- MicroRNA-127 modulates fetal lung development.Physiological genomics · 2009Article
- Short hairpin RNA interference therapy for ischemic heart disease.Circulation · 2008Article
- Hit selection with false discovery rate control in genome-scale RNAi screens.Nucleic acids research · 2008Article
- Functional involvement of Annexin-2 in cAMP induced AQP2 trafficking.Pflugers Archiv : European journal of physiology · 2008Article
- Genome-wide screens for effective siRNAs through assessing the size of siRNA effects.BMC research notes · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we report a cell-specific RNAi system using an alveolar epithelial type II cell-specific promoter--the surfactant protein C (SP-C) promoter. We show that the SP-C-driven small hairpin RNAs specifically depress the expression of the exogenous reporter (enhanced green fluorescent protein) and endogenous genes (lamin A/C and annexin A2) in alveolar type II cells, but not other lung cells, using cell and organ culture in vitro as well as in vivo. The present study provides an efficient strategy in silencing a gene in one type of cell without interfering with other cell systems, and may have a significant impact on RNAi therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.