Evidence map›Paper›PMID 15452148›Full record

ArticleThe Journal of cell biology2004

CD44 modulates Smad1 activation in the BMP-7 signaling pathway.

Richard S Peterson, Roma A Andhare, Kathleen T Rousche, Warren Knudson, Weihua Wang, Jami B Grossfield, Raymond O Thomas, Robert E Hollingsworth, Cheryl B Knudson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 83 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Current and Future Concepts for the Treatment of Impaired Fracture Healing.International journal of molecular sciences · 2019
    Review
  7. Review
  8. The pericellular hyaluronan of articular chondrocytes.Matrix biology : journal of the International Society for Matrix Biology · 2019
    Review
  9. Article
  10. Article
  11. Hyaluronic acid in digestive cancers.Journal of cancer research and clinical oncology · 2017
    Review
  12. Article
  13. Signaling Receptors for TGF-β Family Members.Cold Spring Harbor perspectives in biology · 2016
    Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Richard S PetersonDept. of Biochemistry, Rush Medical College, Rush University Medical Center, 1653 West Congress Parkway, Chicago, IL 60612, USA.
Roma A Andhare
Kathleen T Rousche
Warren Knudson
Weihua Wang
Jami B Grossfield
Raymond O Thomas
Robert E Hollingsworth
Cheryl B Knudson
Rush University Medical Center · USGlaxoSmithKline (United States) · USResearch Triangle Park Foundation · US

Funding

SPECIALIZED CENTER OF RESEARCH IN OSTEOARTHRITISP50AR039239 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI OEGEMA, THEODORE RICHARD · 1987 to 2007
$8.5M
HYALURONAN-CELL INTERACTIONS IN CARTILAGER01AR039507 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI KNUDSON, CHERYL B · 1998 to 2013
$3.1M
CD44 MEDIATED CATABOLISM OF HYALURONAN BY CHONDROCYTESR01AR043384 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI KNUDSON, WARREN · 1996 to 2009
$2.7M
NIAMS NIH HHS P50 AR039239NIAMS NIH HHS P50 AR 39239NIAMS NIH HHS R01 AR039507NIAMS NIH HHS R01 AR043384NIAMS NIH HHS R01 AR 39507NIAMS NIH HHS R01 AR 43384
6 · The paper itself

Abstract

Bone morphogenetic protein 7 (BMP-7) regulates cellular metabolism in embryonic and adult tissues. Signal transduction occurs through the activation of intracellular Smad proteins. In this paper, using a yeast two-hybrid screen, Smad1 was found to interact with the cytoplasmic domain of CD44, a receptor for the extracellular matrix macromolecule hyaluronan. Coimmunoprecipitation experiments confirmed the interaction of Smad1 with full-length CD44-interactions that did not occur when CD44 receptors truncated within the cytoplasmic domain were tested. Chondrocytes overexpressing a truncated CD44 on a background of endogenous full-length CD44 no longer exhibited Smad1 nuclear translocation upon BMP-7 stimulation. Further, pretreatment of chondrocytes with Streptomyces hyaluronidase to disrupt extracellular hyaluronan-cell interactions inhibited BMP-7-mediated Smad1 phosphorylation, nuclear translocation of Smad1 or Smad4, and SBE4-luciferase reporter activation. These results support a functional link between the BMP signaling cascade and CD44. Thus, changes in hyaluronan-cell interactions may serve as a means to modulate cellular responsiveness to BMP.

Indexed as

Active Transport, Cell NucleusAnimalsBone Morphogenetic Protein 7Bone Morphogenetic ProteinsCartilageCattleChondrocytesCOS CellsDNA-Binding ProteinsExtracellular MatrixGenes, ReporterHumansHyaluronan ReceptorsHyaluronic AcidHyaluronoglucosaminidaseMutagenesis, Site-DirectedBMP7 protein, humanBone Morphogenetic Protein 7Bone Morphogenetic ProteinsDNA-Binding ProteinsHyaluronan ReceptorsHyaluronic AcidHyaluronoglucosaminidaseSmad1 ProteinSMAD1 protein, humanSmad4 ProteinSMAD4 protein, humanSmad ProteinsTrans-ActivatorsTranscription FactorsTransforming Growth Factor beta

Identifiers

PMID15452148
PMCPMC2172010
OpenAlexW2088585573

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.