ArticlePlanta2004
Molecular and biochemical characterization of the Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) gene induced by abiotic and biotic stresses.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 72 citations in OpenAlex.
- Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper.BMC plant biology · 2024Article
- New insights into the evolution and functional divergence of the CIPK gene family in Saccharum.BMC genomics · 2020Article
- The Grapevine Calmodulin-Like Protein GeneInternational journal of molecular sciences · 2020Article
- The Effect of Abiotic Stress Conditions on Expression of Calmodulin (Plants (Basel, Switzerland) · 2019Article
- Insights on Calcium-Dependent Protein Kinases (CPKs) Signaling for Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2019Review
- The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane.The Journal of biological chemistry · 2018Article
- Riboflavin-Induced Disease Resistance Requires the Mitogen-Activated Protein Kinases 3 and 6 in Arabidopsis thaliana.PloS one · 2016Article
- Genome-wide analysis and expression of the calcium-dependent protein kinase gene family in cucumber.Molecular genetics and genomics : MGG · 2015Article
- Review
- Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants.Frontiers in plant science · 2015Review
- Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.Nature protocols · 2014Article
- Structure and expression profiling of a novel calcium-dependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr.Plant cell reports · 2013Article
- Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement.Plant physiology · 2007Article
- Vitamin B1 functions as an activator of plant disease resistance.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolated full-length Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) cDNA clone was selected from the chili pepper expressed sequence tag database (http://www.pdrc.re.kr/ks200201/pepper.html). Phylogenetic analysis based on the deduced amino acid sequence of CaCDPK3 cDNA revealed significant sequence similarity to the winter squash (Cucurbita maxima) CmCPK2 gene (81% identity). Genomic gel blot analysis disclosed that CaCDPK3 belongs to a multigene family in the pepper genome. CaCDPK3 expression was root tissue-specific, as shown by Northern blot data. The gene was rapidly induced in response to various osmotic stress factors and exogenous abscisic acid application in pepper leaves. Moreover, CaCDPK3 RNA expression was induced by an incompatible pathogen and by plant defense-related chemicals such as ethephon, salicylic acid and jasmonic acid. The biochemical properties of CaCDPK3 were investigated using a CaCDPK3 and glutathione S-transferase (GST) fusion protein. The recombinant proteins retained calcium-binding ability, and displayed autophosphorylation activity in vitro in a calcium-dependent manner. Further transient-expression studies showed that CaCDPK3 fused with soluble modified green fluorescent protein (smGFP) localized to the cytosol in chili pepper protoplasts. We propose that CaCDPK3 is implicated in biotic and abiotic stresses in pepper plants.
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