ArticleNucleic acids research2004
Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 90 citations in OpenAlex.
- Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.Nucleic acids research · 2021Article
- High-Throughput Generation of In Silico Derived Synthetic Antibodies via One-step Enzymatic DNA Assembly of Fragments.Molecular biotechnology · 2020Article
- A High-Throughput Platform for the Generation of Synthetic Ab Clones by Single-Strand Site-Directed Mutagenesis.Molecular biotechnology · 2019Article
- High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.Nanoscale · 2017Article
- Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
- Express photolithographic DNA microarray synthesis with optimized chemistry and high-efficiency photolabile groups.Journal of nanobiotechnology · 2016Article
- Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Chemical Society reviews · 2015Review
- A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.Nature communications · 2015Article
- Direct mutagenesis of thousands of genomic targets using microarray-derived oligonucleotides.ACS synthetic biology · 2015Article
- Synthetic Biology: A Bridge between Artificial and Natural Cells.Life (Basel, Switzerland) · 2014Review
- Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography.Chemical communications (Cambridge, England) · 2014Article
- Error removal in microchip-synthesized DNA using immobilized MutS.Nucleic acids research · 2014Article
- Large-scale de novo DNA synthesis: technologies and applications.Nature methods · 2014Review
- Emerging tools for synthetic genome design.Molecules and cells · 2013Review
- DNA synthesis, assembly and applications in synthetic biology.Current opinion in chemical biology · 2012Review
- Engineering the third wave of biocatalysis.Nature · 2012Review
- RNA-mediated gene assembly from DNA arrays.Angewandte Chemie (International ed. in English) · 2012Article
- Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays.Journal of nanobiotechnology · 2011Article
- Parallel on-chip gene synthesis and application to optimization of protein expression.Nature biotechnology · 2011Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A basic problem in gene synthesis is the acquisition of many short oligonucleotide sequences needed for the assembly of genes. Photolithographic methods for the massively parallel synthesis of high-density oligonucleotide arrays provides a potential source, once appropriate methods have been devised for their elution in forms suitable for enzyme-catalyzed assembly. Here, we describe a method based on the photolithographic synthesis of long (>60mers) single-stranded oligonucleotides, using a modified maskless array synthesizer. Once the covalent bond between the DNA and the glass surface is cleaved, the full-length oligonucleotides are selected and amplified using PCR. After cleavage of flanking primer sites, a population of unique, internal 40mer dsDNA sequences are released and are ready for use in biological applications. Subsequent gene assembly experiments using this DNA pool were performed and were successful in creating longer DNA fragments. This is the first report demonstrating the use of eluted chip oligonucleotides in biological applications such as PCR and assembly PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.