ArticleBMC microbiology2004
Prediction of DtxR regulon: identification of binding sites and operons controlled by Diphtheria toxin repressor in Corynebacterium diphtheriae.
Article in BMC microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Quinolone Resistance and Zoonotic Potential ofAntibiotics (Basel, Switzerland) · 2025Article
- Genome-Wide Analysis of DtxR and HrrA Regulons Reveals Novel Targets and a High Level of Interconnectivity Between Iron and Heme Regulatory Networks in Corynebacterium glutamicum.Molecular microbiology · 2025Article
- The Exoproteome and Surfaceome of ToxigenicJournal of proteome research · 2025Article
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- Comparative Genomic Analysis of Two Chilean Renibacterium salmoninarum Isolates and the Type Strain ATCC 33209T.Genome biology and evolution · 2018Article
- Bacterial Stress Responses during Host Infection.Cell host & microbe · 2016Review
- An affinity-structure database of helix-turn-helix: DNA complexes with a universal coordinate system.BMC bioinformatics · 2015Article
- TiO2 photocatalysis damages lipids and proteins in Escherichia coli.Applied and environmental microbiology · 2014Article
- A role for the DtxR family of metalloregulators in gram-positive pathogenesis.Molecular oral microbiology · 2014Review
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- Evaluating phylogenetic congruence in the post-genomic era.Genome biology and evolution · 2011Review
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- Development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria.FEMS microbiology letters · 2009Article
- Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae.Molecular microbiology · 2007Article
- MycoperonDB: a database of computationally identified operons and transcriptional units in Mycobacteria.BMC bioinformatics · 2006Article
- iCR: a web tool to identify conserved targets of a regulatory protein across the multiple related prokaryotic species.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe diphtheria toxin repressor, DtxR, of Corynebacterium diphtheriae has been shown to be an iron-activated transcription regulator that controls not only the expression of diphtheria toxin but also of iron uptake genes. This study aims to identify putative binding sites and operons controlled by DtxR to understand the role of DtxR in patho-physiology of Corynebacterium diphtheriae.
resultPositional Shannon relative entropy method was used to build the DtxR-binding site recognition profile and the later was used to identify putative regulatory sites of DtxR within C. diphtheriae genome. In addition, DtxR-regulated operons were also identified taking into account the predicted DtxR regulatory sites and genome annotation. Few of the predicted motifs were experimentally validated by electrophoretic mobility shift assay. The analysis identifies motifs upstream to the novel iron-regulated genes that code for Formamidopyrimidine-DNA glycosylase (FpG), an enzyme involved in DNA-repair and starvation inducible DNA-binding protein (Dps) which is involved in iron storage and oxidative stress defense. In addition, we have found the DtxR motifs upstream to the genes that code for sortase which catalyzes anchoring of host-interacting proteins to the cell wall of pathogenic bacteria and the proteins of secretory system which could be involved in translocation of various iron-regulated virulence factors including diphtheria toxin.
conclusionsWe have used an in silico approach to identify the putative binding sites and genes controlled by DtxR in Corynebacterium diphtheriae. Our analysis shows that DtxR could provide a molecular link between Fe+2-induced Fenton's reaction and protection of DNA from oxidative damage. DtxR-regulated Dps prevents lethal combination of Fe+2 and H2O2 and also protects DNA by nonspecific DNA-binding. In addition DtxR could play an important role in host interaction and virulence by regulating the levels of sortase, a potential vaccine candidate and proteins of secretory system.
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