ArticleJournal of neurochemistry2004
Proteomic analysis of native metabotropic glutamate receptor 5 protein complexes reveals novel molecular constituents.
Article in Journal of neurochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Synaptic proteomics decode novel molecular landscape in the brain.Frontiers in molecular neuroscience · 2024Review
- Cell-Type-Specific Neuroproteomics of Synapses.Biomolecules · 2023Review
- A unified resource and configurable model of the synapse proteome and its role in disease.Scientific reports · 2021Article
- Proteomic insights into synaptic signaling in the brain: the past, present and future.Molecular brain · 2021Review
- Functionally selective activation of the dopamine receptor DScientific reports · 2021Article
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Temporal dynamics of miRNAs in human DLPFC and its association with miRNA dysregulation in schizophrenia.Translational psychiatry · 2019Article
- Proteomics in the Diagnosis of Inborn Encephalopathies of Unknown Origin: A Myth or Reality.Advances in experimental medicine and biology · 2018Review
- Article
- Synaptic Interactome Mining Reveals p140Cap as a New Hub for PSD Proteins Involved in Psychiatric and Neurological Disorders.Frontiers in molecular neuroscience · 2017Article
- Group 1 metabotropic glutamate receptors 1 and 5 form a protein complex in mouse hippocampus and cortex.Proteomics · 2016Article
- Regulation of neuronal chloride homeostasis by neuromodulators.The Journal of physiology · 2016Review
- Proteomics of the Synapse--A Quantitative Approach to Neuronal Plasticity.Molecular & cellular proteomics : MCP · 2016Review
- Human cognitive ability is influenced by genetic variation in components of postsynaptic signalling complexes assembled by NMDA receptors and MAGUK proteins.Translational psychiatry · 2014Article
- Identification of GPCR-interacting cytosolic proteins using HDL particles and mass spectrometry-based proteomic approach.PloS one · 2013Article
- Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum.The Journal of biological chemistry · 2012Article
- Article
- Genes and pathways co-associated with the exposure to multiple drugs of abuse, including alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine, and/or nicotine: a review of proteomics analyses.Molecular neurobiology · 2011Review
- A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.Molecular & cellular proteomics : MCP · 2011Article
- Ex vivo identification of protein-protein interactions involving the dopamine transporter.Journal of neuroscience methods · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
We used a proteomic approach to identify novel proteins that may regulate metabotropic glutamate receptor 5 (mGluR5) responses by direct or indirect protein interactions. This approach does not rely on the heterologous expression of proteins and offers the advantage of identifying protein interactions in a native environment. The mGluR5 protein was immunoprecipitated from rat brain lysates; co-immunoprecipitating proteins were analyzed by mass spectrometry and identified peptides were matched to protein databases to determine the correlating parent proteins. This proteomic approach revealed the interaction of mGluR5 with known regulatory proteins, as well as novel proteins that reflect previously unidentified molecular constituents of the mGluR5-signaling complex. Immunoblot analysis confirmed the interaction of high confidence proteins, such as phosphofurin acidic cluster sorting protein 1, microtubule-associated protein 2a and dynamin 1, as mGluR5-interacting proteins. These studies show that a proteomic approach can be used to identify candidate interacting proteins. This approach may be particularly useful for neurobiology applications where distinct protein interactions within a signaling complex can dramatically alter the outcome of the response to neurotransmitter release, or the disruption of normal protein interactions can lead to severe neurological and psychiatric disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.