ArticleMammalian genome : official journal of the International Mammalian Genome Society1992
Rapid isolation of desired sequences from lone linker PCR amplified cDNA mixtures: application to identification and recovery of expressed sequences in cloned genomic DNA.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 29 citations in OpenAlex.
- Review
- DNA enrichment by allele-specific hybridization (DEASH): a novel method for haplotyping and for detecting low-frequency base substitutional variants and recombinant DNA molecules.Genome research · 2003Article
- Article
- A systematic molecular genetic approach to study mammalian germline development.The International journal of developmental biology · 1998Review
- Coincidence cloning. Taking the coincidences out of genome analysis.Molecular biotechnology · 1996Review
- Monitoring the efficacy of hybrid selection during positional cloning: the search for BRCA1.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
- Identifying genes within microdissected genomic DNA: isolation of brain expressed genes from a translocation region associated with inherited mental illness.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
- Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Efficiency and specificity of gene isolation by exon amplification.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
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1 author at 1 institution in 1 country.
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Abstract
A simple and efficient method for the rapid isolation of specific sequences from PCR-amplified cDNA mixtures has been developed. cDNA mixtures obtained using lone linker PCR (Ko et al. 1990) appeared to be highly representative even though the starting material, 100 ng-2 micrograms of total RNA, is much less than is required for making an ordinary cDNA library. With this method, cDNA mixtures were obtained from limited materials, including early mouse embryos and primordial germ cells. For selective enrichment of desired cDNAs, biotinylated probe was hybridized with the lone linker-linked cDNA in solution and the resulting probe-cDNA hybrid was captured by Streptavidin-coated magnetic beads. After appropriate washing, cDNA was released from the beads and subjected to amplification followed by cloning into a vector. Using genomic fragments isolated during chromosomal walking in the T/t complex of mouse Chromosome (Chr) 17, cDNAs encoding novel germ cell specific genes have been readily isolated by the above procedures. The method, termed random access retrieval of genetic information through PCR (RARGIP), will streamline the entire process from RNA to cDNA greatly. Its application potentials in various areas of molecular genetics will be discussed.
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