ArticleThe Journal of cell biology1992
An interaction between zyxin and alpha-actinin.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 254 citations in OpenAlex.
- IgA autoantibodies against human eye muscle antigen detected by western blotting and immunohistochemical methods in Graves' disease.Journal of endocrinological investigation · 1995Trial
- Co-Immunoprecipitation-Coupled Mass Spectrometry Analysis of Zyxin's Interactome and Phosphosites in EarlyInternational journal of molecular sciences · 2026Article
- Structural and functional insights into α-actinin isoforms and their implications in cardiovascular disease.The Journal of general physiology · 2025Review
- Down Expression of Zyxin is Associated with Down Expression of p53 in Colorectal Cancer.International journal of molecular and cellular medicine · 2025Article
- Article
- Alteration of actin cytoskeletal organisation in fetal akinesia deformation sequence.Scientific reports · 2024Article
- Exploring Co-expression Modules-Traits Correlation through Weighted Gene Co-expression Network Analysis: A Promising Approach in Wound Healing Research.Medical journal of the Islamic Republic of Iran · 2024Article
- Review
- A Layered View on Focal Adhesions.Biology · 2021Review
- Spatial proximity of proteins surrounding zyxin under force-bearing conditions.Molecular biology of the cell · 2021Article
- Actin Cytoskeleton and Regulation of TGFβ Signaling: Exploring Their Links.Biomolecules · 2021Review
- Zyxin Mediates Vascular RepairFrontiers in physiology · 2021Article
- Mechanosensing through Direct Binding of Tensed F-Actin by LIM Domains.Developmental cell · 2020Article
- Overview of the Muscle Cytoskeleton.Comprehensive Physiology · 2017Review
- Global Analysis of mRNA, Translation, and Protein Localization: Local Translation Is a Key Regulator of Cell Protrusions.Developmental cell · 2015Article
- Mechanical dynamics in live cells and fluorescence-based force/tension sensors.Biochimica et biophysica acta · 2015Review
- Loss of the mechanotransducer zyxin promotes a synthetic phenotype of vascular smooth muscle cells.Journal of the American Heart Association · 2015Article
- α-Actinin-4 enhances colorectal cancer cell invasion by suppressing focal adhesion maturation.PloS one · 2015Article
- The conserved LIM domain-containing focal adhesion protein ZYX-1 regulates synapse maintenance in Caenorhabditis elegans.Development (Cambridge, England) · 2014Article
- The nebulin SH3 domain is dispensable for normal skeletal muscle structure but is required for effective active load bearing in mouse.Journal of cell science · 2013Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zyxin is an 82-kD protein first identified as a component of adhesion plaques and the termini of stress fibers near where they associate with the cytoplasmic face of the adhesive membrane. We report here that zyxin interacts with the actin cross-linking protein alpha-actinin. Zyxin cosediments with filamentous actin in an alpha-actinin-dependent manner and an association between zyxin and alpha-actinin is observed in solution by analytical gel filtration. The specificity of the interaction between zyxin and alpha-actinin was demonstrated by blot overlay experiments in which 125I-zyxin recognizes most prominently alpha-actinin among a complex mixture of proteins extracted from avian smooth muscle. By these blot overlay binding studies, we determined that zyxin interacts with the NH2-terminal 27-kD domain of alpha-actinin, a region that also contains the actin binding site. Solid phase binding assays were performed to evaluate further the specificity of the binding and to determine the affinity of the zyxin-alpha-actinin interaction. By these approaches we have demonstrated a specific, saturable, moderate-affinity interaction between zyxin and alpha-actinin. Furthermore, double-label immunofluorescence reveals that zyxin and alpha-actinin exhibit extensive overlap in their subcellular distributions in both chicken embryo fibroblasts and pigmented retinal epithelial cells. The significant colocalization of the two proteins is consistent with the possibility that the interaction between zyxin and alpha-actinin has a biologically relevant role in coordinating membrane-cytoskeletal interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.