ArticleThe Journal of cell biology1992
The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 99 citations in OpenAlex.
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- Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.Journal of biological methods · 2018Article
- The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.The Journal of cell biology · 2006Article
- Article
- Electrophysiological analysis of the yeast V-type proton pump: variable coupling ratio and proton shunt.Biophysical journal · 2003Article
- Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- Cnm67p is a spacer protein of the Saccharomyces cerevisiae spindle pole body outer plaque.Molecular biology of the cell · 2001Article
- The Tpr protein: linking structure and function in the nuclear interior?American journal of human genetics · 1998Review
- Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis.Molecular and cellular biology · 1998Article
- Purification, characterization and molecular cloning of TGP1, a novel G-DNA binding protein from Tetrahymena thermophila.Nucleic acids research · 1998Article
- A mutational analysis identifies three functional regions of the spindle pole component Spc110p in Saccharomyces cerevisiae.Molecular biology of the cell · 1997Article
- Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p.The EMBO journal · 1997Article
- A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex.Molecular and cellular biology · 1996Article
- MFP1, a novel plant filament-like protein with affinity for matrix attachment region DNA.The Plant cell · 1996Article
- Spc110p: assembly properties and role in the connection of nuclear microtubules to the yeast spindle pole body.The EMBO journal · 1996Article
- The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment.The EMBO journal · 1996Article
- Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p.Molecular biology of the cell · 1996Article
- Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents.The Journal of cell biology · 1996Article
- Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication.The Journal of cell biology · 1996Article
- The 110-kD spindle pole body component of Saccharomyces cerevisiae is a phosphoprotein that is modified in a cell cycle-dependent manner.The Journal of cell biology · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In an attempt to identify structural components of the yeast nucleus, subcellular fractions of yeast nuclei were prepared and used as immunogens to generate complex polyclonal antibodies. One such serum was used to screen a yeast genomic lambda gt11 expression library. A clone encoding a gene called NUF1 (for nuclear filament-related) was identified and extensively characterized. Antibodies to NUF1 fusion proteins were generated, and affinity-purified antibodies were used for immunoblot analysis and indirect immunofluorescence localization. The NUF1 protein is 110 kD in molecular mass and localizes to the yeast nucleus in small granular patches. Intranuclear staining is present in cells at all stages of the cell cycle. The NUF1 protein of yeast is tightly associated with the nucleus; it was not removed by extraction of nuclei with nonionic detergent or salt, or treatment with RNAse and DNAse. Sequence analysis of the NUF1 gene predicts a protein 945 amino acids in length that contains three domains: a large 627 residue central domain predicted to form a coiled-coil structure flanked by nonhelical amino-terminal and carboxy-terminal regions. Disruption of the NUF1 gene indicates that it is necessary for yeast cell growth. These results indicate that NUF1 encodes an essential coiled-coil protein within the yeast nucleus; we speculate that NUF1 is a component of the yeast nucleoskeleton. In addition, immunofluorescence results indicate that mammalian cells contain a NUF1-related nuclear protein. These data in conjunction with those in the accompanying manuscript (Yang et al., 1992) lead to the hypothesis that an internal coiled-coil filamentous system may be a general structural component of the eukaryotic nucleus.
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