ArticleInfection and immunity1992
Movement along actin filaments of the perijunctional area and de novo polymerization of cellular actin are required for Shigella flexneri colonization of epithelial Caco-2 cell monolayers.
Article in Infection and immunity, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Bacterial spread from cell to cell: beyond actin-based motility.Trends in microbiology · 2015Review
- Article
- Article
- VirB-mediated positive feedback control of the virulence gene regulatory cascade of Shigella flexneri.Journal of bacteriology · 2012Article
- Distinct roles of secreted HtrA proteases from gram-negative pathogens in cleaving the junctional protein and tumor suppressor E-cadherin.The Journal of biological chemistry · 2012Article
- The pathogenesis of Shigella flexneri infection: lessons from in vitro and in vivo studies.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Isolation of Neisseria gonorrhoeae mutants that show enhanced trafficking across polarized T84 epithelial monolayers.Infection and immunity · 2000Article
- Outer membrane protein A-promoted actin condensation of brain microvascular endothelial cells is required for Escherichia coli invasion.Infection and immunity · 1999Article
- Vaccination against shigellosis with attenuated Shigella flexneri 2a strain SC602.Infection and immunity · 1999Article
- Diarrheagenic Escherichia coli.Clinical microbiology reviews · 1998Review
- Human intestinal epithelial cells swell and demonstrate actin rearrangement in response to the metalloprotease toxin of Bacteroides fragilis.Infection and immunity · 1996Article
- Gonococcal opacity protein promotes bacterial entry-associated rearrangements of the epithelial cell actin cytoskeleton.Infection and immunity · 1996Article
- Regulation of surface presentation of IcsA, a Shigella protein essential to intracellular movement and spread, is growth phase dependent.Infection and immunity · 1994Article
- Construction and characterization of attenuated delta aroA delta virG Shigella flexneri 2a strain CVD 1203, a prototype live oral vaccine.Infection and immunity · 1994Article
- Acute inflammation causes epithelial invasion and mucosal destruction in experimental shigellosis.The Journal of experimental medicine · 1994Article
- Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence.Journal of bacteriology · 1994Article
- Shigella subversion of the cellular cytoskeleton: a strategy for epithelial colonization.Infection and immunity · 1993Review
- Bacterial pathogens, from adherence to invasion: comparative strategies.Medical microbiology and immunology · 1993Review
- Directional actin polymerization associated with spotted fever group Rickettsia infection of Vero cells.Infection and immunity · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella flexneri invades eucaryotic cells and grows in the cytoplasm. Lysis of the phagosomal membrane is a prerequisite for both intracellular multiplication and movement of the bacteria that gain direct access to the host cell actin. In HeLa cells, bacteria generate their own movement essentially by inducing actin polymerization. Polymerization of actin enables them to move rapidly and randomly in the cytoplasm and to spread from one cell to another through protrusions of the host cell membrane. This movement was designated the Ics phenotype. In contrast, in chicken embryo fibroblasts, bacteria move along actin filaments in a very organized manner, following the cytoskeletal architecture; this movement was designated the Olm phenotype. Bacterial movement is a major virulence factor in that it is necessary for efficient colonization of the intestinal epithelium of infected macaque monkeys. Further characterization of the cellular events that lead to colonization of the colonic intestinal epithelium was needed. In order to characterize the movement in vitro in a cell assay system more closely related to the intestinal epithelium, we used human colonic epithelial Caco-2 cells. The movement of bacteria as observed by using immunofluorescence and confocal microscopy appeared to result from the expression of both the Olm and Ics phenotypes. The former allowed colonization of cells along the actin filament ring of the perijunctional area. The latter promoted passage from one cell to adjacent cells. This in vitro pattern of movement and multiplication gives S. flexneri, once it has entered an epithelial cell, the unique capacity to spread through the entire epithelial layer without having further contact with the extracellular compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.