ArticleBiophysical journal1992
Lateral mobility of integral proteins in red blood cell tethers.
Article in Biophysical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- The Double-Edged Sword of Erythrocytes in Health and Disease via Their Adhesiveness.International journal of molecular sciences · 2023Review
- Red Blood Cells: Tethering, Vesiculation, and Disease in Micro-Vascular Flow.Diagnostics (Basel, Switzerland) · 2021Article
- Membrane curvature regulates ligand-specific membrane sorting of GPCRs in living cells.Nature chemical biology · 2017Article
- Membrane Mechanics of Primary Afferent Neurons in the Dorsal Root Ganglia of Rats.Biophysical journal · 2017Article
- Analysis of diffusion in curved surfaces and its application to tubular membranes.Molecular biology of the cell · 2016Article
- Diffusion of glycophorin A in human erythrocytes.Biochimica et biophysica acta · 2016Article
- The properties of chondrocyte membrane reservoirs and their role in impact-induced cell death.Biophysical journal · 2013Article
- Cell cytoskeleton and tether extraction.Biophysical journal · 2011Article
- Cell membrane tethers generate mechanical force in response to electrical stimulation.Biophysical journal · 2010Article
- Multiscale simulation of erythrocyte membranes.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Role of E-cadherin in membrane-cortex interaction probed by nanotube extrusion.Biophysical journal · 2009Article
- Integral protein linkage and the bilayer-skeletal separation energy in red blood cells.Biophysical journal · 2008Article
- Actin cytoskeleton-dependent dynamics of the human serotonin1A receptor correlates with receptor signaling.Biophysical journal · 2008Article
- Tether extrusion from red blood cells: integral proteins unbinding from cytoskeleton.Biophysical journal · 2007Article
- Function, expression and localization of annexin A7 in platelets and red blood cells: insights derived from an annexin A7 mutant mouse.BMC biochemistry · 2003Article
- Translational diffusion of individual class II MHC membrane proteins in cells.Biophysical journal · 2002Article
- Membrane tether formation from blebbing cells.Biophysical journal · 1999Article
- Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.The Journal of cell biology · 1999Article
- Membrane dynamics of the water transport protein aquaporin-1 in intact human red cells.Biophysical journal · 1999Article
- Regulation mechanism of the lateral diffusion of band 3 in erythrocyte membranes by the membrane skeleton.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The red blood cell membrane is a complex material that exhibits both solid- and liquidlike behavior. It is distinguished from a simple lipid bilayer capsule by its mechanical properties, particularly its shear viscoelastic behavior and by the long-range mobility of integral proteins on the membrane surface. Subject to sufficiently large extension, the membrane loses its shear rigidity and flows as a two-dimensional fluid. These experiments examine the change in integral protein mobility that accompanies the mechanical phenomenon of extensional failure and liquidlike flow. A flow channel apparatus is used to create red cell tethers, hollow cylinders of greatly deformed membrane, up to 36-microns long. The diffusion of proteins within the surface of the membrane is measured by the technique of fluorescence redistribution after photobleaching (FRAP). Integral membrane proteins are labeled directly with a fluorescein dye (DTAF). Mobility in normal membrane is measured by photobleaching half of the cell and measuring the rate of fluorescence recovery. Protein mobility in tether membrane is calculated from the fluorescence recovery rate after the entire tether has been bleached. Fluorescence recovery rates for normal membrane indicate that more than half the labeled proteins are mobile with a diffusion coefficient of approximately 4 x 10(-11) cm2/s, in agreement with results from other studies. The diffusion coefficient for proteins in tether membrane is greater than 1.5 x 10(-9) cm2/s. This dramatic increase in diffusion coefficient indicates that extensional failure involves the uncoupling of the lipid bilayer from the membrane skeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.