ArticleProtein science : a publication of the Protein Society2004
Arm-domain interactions can provide high binding cooperativity.
Article in Protein science : a publication of the Protein Society, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- A DNA-assisted binding assay for weak protein-protein interactions.Journal of molecular biology · 2009Article
- Linkage-specific avidity defines the lysine 63-linked polyubiquitin-binding preference of rap80.Molecular cell · 2009Article
- Extending Iterative Protein Redesign and Optimization (IPRO) in protein library design for ligand specificity.Biophysical journal · 2007Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Peptidyl arms extending from one protein domain to another protein domain mediate many important interactions in biology. A well-studied example of this type of protein-protein interaction occurs between the yeast homeodomain proteins, MAT alpha2 and MAT a1, which form a high-affinity heterodimer on DNA. The carboxyl-terminal arm extending from MAT alpha2 to MAT a1 has been proposed to produce an allosteric conformational change in the a1 protein that generates a very large increase in the DNA binding affinity of a1. Although early studies lent some support to this model, a more recent crystal structure determination of the free a1 protein argues against any allosteric change. This note presents a thermodynamic argument that accounts for the proteins' binding behavior, so that allosteric conformational changes are not required to explain the large affinity increase. The analysis presented here should be useful in analyzing binding behavior in other systems involving arm interactions.
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Registered trials
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