Evidence map›Paper›PMID 15387328›Full record

ArticleDigestive diseases and sciences2004

Probiotics reduce bacterial colonization and gastric inflammation in H. pylori-infected mice.

Kathene Candace Johnson-Henry, David J Mitchell, Yaron Avitzur, Esther Galindo-Mata, Nicola L Jones, Philip M Sherman

Abstract read
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In one paragraph

Article in Digestive diseases and sciences, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed, 3 pooled it
8.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 3 syntheses or guidelines pooled it, 154 citations in OpenAlex.

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  15. Article
  16. Efficacy ofAntibiotics (Basel, Switzerland) · 2022
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  17. Effect of a Probiotic Mixture in Captive Cheetahs (Animals : an open access journal from MDPI · 2022
    Article
  18. The interactions between oral-gut axis microbiota andFrontiers in cellular and infection microbiology · 2022
    Review
  19. Article
  20. Curcumin Oxidation Is Required for Inhibition ofFrontiers in cellular and infection microbiology · 2021
    Article

5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Kathene Candace Johnson-HenryUniversity of Toronto, Research Institute, Hospital for Sick Children, Department of Gastroenterology and Nutrition, Infection, Immunity, Injury and Repair, Toronto, Ontario, Canada. kathene.johnson-henry@sickkids.ca
David J Mitchell
Yaron Avitzur
Esther Galindo-Mata
Nicola L Jones
Philip M Sherman
University of Toronto · CASickKids Foundation · CAHospital for Sick Children · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Probiotics are characterized by their ability to interact with commensal microflora in the gastrointestinal tract to produce beneficial health effects. In vitro studies suggest that Lactobacillus species have the potential to suppress the growth of Helicobacter pylori. The goal of this study was to determine if pretreatment of mice with a commercial mixture of live probiotics (L. rhamnosus, strain R0011, and L. acidophilus, strain R0052) would suppress colonization of H. pylori, strain SS1. Thirty C57BL/6 female mice were divided into four groups: Group A was fed sterile water, group B received probiotics in sterile drinking water, group C was challenged orogastrically with H. pylori, and group D was pretreated with probiotics in drinking water prior to and following challenge with H. pylori. Rectal swabs, stomach homogenates, and luminal contents from ileum and colon were plated onto colistin nalidixic acid plates. Serial dilutions of stomach homogenates were plated onto H. pylori-sensitive agar plates and incubated under microaerophilic conditions. Tissue samples from the stomach were analyzed histologically to determine the degree of H. pylori colonization, mucosal inflammation, and epithelial cell apoptosis. Probiotics in drinking water did not affect the overall well-being of mice. Lactobacillus species were excreted in stools over the entire duration of treatment. Pretreatment with probiotics reduced the number of mice with H. pylori growth from stomach homgenates (100 to 50%; P = 0.02). The percentage of mice with moderate-severe H. pylori-induced inflammation in the gastric antrum was reduced with probiotic pretreatment (71 to 29%; P = 0.14). However, pretreatment with probiotics did not prevent H. pylori-induced apoptosis in the gastric mucosa. This preparation of probiotics provided a safe and novel approach for reducing H. pylori colonization and bacterial-induced inflammation of mice.

Indexed as

Helicobacter pyloriLactobacillusAnimalsApoptosisFemaleGastritisHelicobacter InfectionsIn Situ Nick-End LabelingMiceMice, Inbred C57BLProbiotics

Identifiers

PMID15387328
OpenAlexW1986328441

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.