ReviewGene therapy2004
Gene therapy progress and prospects: episomally maintained self-replicating systems.
Review in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Harnessing the central dogma: Enzyme-driven gene diversification strategies for in cellulo continuous directed evolution.Fundamental research · 2026Review
- Molecular Mechanisms in Pathophysiology of Mucopolysaccharidosis and Prospects for Innovative Therapy.International journal of molecular sciences · 2024Review
- Current strategies employed in the manipulation of gene expression for clinical purposes.Journal of translational medicine · 2022Review
- Diseased, differentiated and difficult: Strategies for improved engineering ofFrontiers in cellular neuroscience · 2022Review
- Prospects of Non-Coding Elements in Genomic DNA Based Gene Therapy.Current gene therapy · 2022Review
- Expanding the genetic code of the human hematopoietic system.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Sustained gene expression in the retina by improved episomal vectors.Tissue engineering. Part A · 2014Article
- The future of human DNA vaccines.Journal of biotechnology · 2012Review
- Use of human MAR elements to improve retroviral vector production.Gene therapy · 2011Article
- Stable S/MAR-based episomal vectors are regulated at the chromatin level.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Dominant-negative effects of COL7A1 mutations can be rescued by controlled overexpression of normal collagen VII.The Journal of biological chemistry · 2009Article
- Robust in vivo transduction of a genetically stable Epstein-Barr virus episome to hepatocytes in mice by a hybrid viral vector.Journal of virology · 2009Article
- Interspecific transfer of mammalian artificial chromosomes between farm animals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Intrathecal injection of naked plasmid DNA provides long-term expression of secreted proteins.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector.Gene therapy · 2008Article
- E2F in vivo binding specificity: comparison of consensus versus nonconsensus binding sites.Genome research · 2008Article
- An S/MAR-based infectious episomal genomic DNA expression vector provides long-term regulated functional complementation of LDLR deficiency.Nucleic acids research · 2007Article
- Genetically modified pigs produced with a nonviral episomal vector.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Plasmid engineering for controlled and sustained gene expression for nonviral gene therapy.Pharmaceutical research · 2006Review
- Increased missegregation and chromosome loss with decreasing chromosome size in vertebrate cells.Chromosoma · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of nonviral gene therapy vectors has been hampered by low level of transfection efficiency and lack of sustained gene expression. Episomal self-replicating systems may overcome these hurdles through their large packaging capacity, stability and reduced toxicity. This article reviews three classes of episomal molecules that have been tested with possible therapeutic genes: (1) self-replicating circular vectors, containing the Epstein-Barr virus (EBV) elements oriP and EBNA1; (2) small circular vectors containing scaffold/matrix attachment regions (S/MARs) as cis-acting elements to maintain the episomal status of the vector; (3) chromosomal vectors, based on the functional elements of the natural chromosomes. The studies reported validate the use of episomal vectors to obtain stable and prolonged gene expression, although reveal some limitations that necessitate additional work.
Indexed as
Identifiers
15385951What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.