ArticleInfection and immunity2004
A Vibrio cholerae classical TcpA amino acid sequence induces protective antibody that binds an area hypothesized to be important for toxin-coregulated pilus structure.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Strain-dependentFrontiers in microbiology · 2026Article
- Variations in the Antivirulence Effects of Fatty Acids and Virstatin againstJournal of microbiology and biotechnology · 2024Article
- Expression of Cholera Toxin (CT) and the Toxin Co-Regulated Pilus (TCP) by Variants of ToxT inToxins · 2023Article
- Host-derived O-glycans inhibit toxigenic conversion by a virulence-encoding phage in Vibrio cholerae.The EMBO journal · 2023Article
- InactivatedJournal of microbiology and biotechnology · 2022Article
- Transcriptional profiling of Vibrio cholerae O1 following exposure to human anti- lipopolysaccharide monoclonal antibodies.Pathogens and disease · 2020Article
- Cholera Toxin Production inFrontiers in microbiology · 2020Article
- Replication ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Recombinant toxin-coregulated pilus A (TcpA) as a candidate subunit cholera vaccine.Iranian journal of microbiology · 2014Article
- Lipopolysaccharide modifications of a cholera vaccine candidate based on outer membrane vesicles reduce endotoxicity and reveal the major protective antigen.Infection and immunity · 2013Article
- Characterization of two outer membrane proteins, FlgO and FlgP, that influence vibrio cholerae motility.Journal of bacteriology · 2009Article
- Conserved regions from Neisseria gonorrhoeae pilin are immunosilent and not immunosuppressive.Infection and immunity · 2007Article
- FppA, a novel Pseudomonas aeruginosa prepilin peptidase involved in assembly of type IVb pili.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Vibrio cholerae is a gram-negative bacterium that has been associated with cholera pandemics since the early 1800s. Whole-cell, killed, and live-attenuated oral cholera vaccines are in use. We and others have focused on the development of a subunit cholera vaccine that features standardized epitopes from various V. cholerae macromolecules that are known to induce protective antibody responses. TcpA protein is assembled into toxin-coregulated pilus (TCP), a type IVb pilus required for V. cholerae colonization, and thus is a strong candidate for a cholera subunit vaccine. Polypeptides (24 to 26 amino acids) in TcpA that can induce protective antibody responses have been reported, but further characterization of their amino acid targets relative to tertiary or quaternary TCP structures has not been done. We report a refinement of the TcpA sequences that can induce protective antibody. One sequence, TcpA 15 (residues 170 to 183), induces antibodies that bind linear TcpA in a Western blot as well as weakly bind soluble TcpA in solution. These antibodies bind assembled pili at high density and provide 80 to 100% protection in the infant mouse protection assay. This is in sharp contrast to other anti-TcpA peptide sera (TcpA 11, TcpA 13, and TcpA 17) that bind very strongly in Western blot and solution assays yet do not provide protection or effectively bind TCP, as evidenced by immunoelectron microscopy. The sequences of TcpA 15 that induce protective antibody were localized on a model of assembled TCP. These sequences are centered on a site that is predicted to be important for TCP structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.