ArticleInfection and immunity2004
Legionella pneumophila DotU and IcmF are required for stability of the Dot/Icm complex.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 95 citations in OpenAlex.
- Article
- The Competitive Edge: T6SS-Mediated Interference Competition byMicroorganisms · 2025Review
- Structure and Function of the Dot/Icm T4SS.bioRxiv : the preprint server for biology · 2023Article
- Comparative Transcriptomics and Genomics from Continuous Axenic Media Growth IdentifiesbioRxiv : the preprint server for biology · 2023Article
- The Missing Pieces: The Role of Secretion Systems inBiomolecules · 2023Review
- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
- Article
- Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system.Microbiology and immunology · 2022Review
- Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker BacteriumGenes · 2021Article
- A ROD9 island encoded gene in Salmonella Enteritidis plays an important role in acid tolerance response and helps in systemic infection in mice.Virulence · 2020Article
- Epidemiology and molecular characteristics of the type VI secretion system in Klebsiella pneumoniae isolated from bloodstream infections.Journal of clinical laboratory analysis · 2020Article
- Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species.Genome biology and evolution · 2019Article
- Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization.The Journal of infectious diseases · 2019Article
- Legionella pneumophila p45 element influences host cell entry and sensitivity to sodium.PloS one · 2019Article
- Integration of Transcriptomic and Proteomic Approaches Reveals the Temperature-Dependent Virulence ofFrontiers in cellular and infection microbiology · 2018Article
- Polar delivery ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Spatiotemporal regulation of a Legionella pneumophila T4SS substrate by the metaeffector SidJ.PLoS pathogens · 2015Article
- The icmF3 locus is involved in multiple adaptation- and virulence-related characteristics in Pseudomonas aeruginosa PAO1.Frontiers in cellular and infection microbiology · 2015Article
- DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli.Frontiers in microbiology · 2014Article
- In depth analysis of the Helicobacter pylori cag pathogenicity island transcriptional responses.PloS one · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Legionella pneumophila utilizes a type IV secretion system (T4SS) encoded by 26 dot/icm genes to replicate inside host cells and cause disease. In contrast to all other L. pneumophila dot/icm genes, dotU and icmF have homologs in a wide variety of gram-negative bacteria, none of which possess a T4SS. Instead, dotU and icmF orthologs are linked to a locus encoding a conserved cluster of proteins designated IcmF-associated homologous proteins, which has been proposed to constitute a novel cell surface structure. We show here that dotU is partially required for L. pneumophila intracellular growth, similar to the known requirement for icmF. In addition, we show that dotU and icmF are necessary for optimal plasmid transfer and sodium sensitivity, two additional phenotypes associated with a functional Dot/Icm complex. We found that these effects are due to the destabilization of the T4SS at the transition into the stationary phase, the point at which L. pneumophila becomes virulent. Specifically, three Dot proteins (DotH, DotG, and DotF) exhibit decreased stability in a DeltadotU DeltaicmF strain. Furthermore, overexpression of just one of these proteins, DotH, is sufficient to suppress the intracellular growth defect of the DeltadotU DeltaicmF mutant. This suggests a model where the DotU and IcmF proteins serve to prevent DotH degradation and therefore function to stabilize the L. pneumophila T4SS. Due to their wide distribution among bacterial species and their genetic linkage to known or predicted cell surface structures, we propose that this function in complex stabilization may be broadly conserved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.