ArticleInfection and immunity2004
IcmF and DotU are required for optimal effector translocation and trafficking of the Legionella pneumophila vacuole.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Structure and Function of the Dot/Icm T4SS.bioRxiv : the preprint server for biology · 2023Article
- Comparative Transcriptomics and Genomics from Continuous Axenic Media Growth IdentifiesbioRxiv : the preprint server for biology · 2023Article
- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
- Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system.Microbiology and immunology · 2022Review
- Viewing Legionella pneumophila Pathogenesis through an Immunological Lens.Journal of molecular biology · 2019Review
- Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization.The Journal of infectious diseases · 2019Article
- Beyond Paralogs: The Multiple Layers of Redundancy in Bacterial Pathogenesis.Frontiers in cellular and infection microbiology · 2017Review
- Iron Limitation Triggers Early Egress by the Intracellular Bacterial Pathogen Legionella pneumophila.Infection and immunity · 2016Article
- A view to a kill: the bacterial type VI secretion system.Cell host & microbe · 2014Review
- Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity.PLoS pathogens · 2013Article
- Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems.The Journal of biological chemistry · 2012Article
- The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC.MicrobiologyOpen · 2012Article
- DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity.PloS one · 2012Article
- Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain.Microbiology (Reading, England) · 2011Article
- Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria.Frontiers in microbiology · 2011Article
- Pore formation triggered by Legionella spp. is an Nlrc4 inflammasome-dependent host cell response that precedes pyroptosis.Infection and immunity · 2010Article
- Legionella pneumophila Dot/Icm translocated substrates: a sum of parts.Current opinion in microbiology · 2009Review
- Large-scale identification of Legionella pneumophila Dot/Icm substrates that modulate host cell vesicle trafficking pathways.Cellular microbiology · 2009Article
- The Legionella pneumophila replication vacuole: making a cosy niche inside host cells.Nature reviews. Microbiology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gram-negative bacterium Legionella pneumophila causes a severe form of pneumonia called Legionnaires' disease, characterized by bacterial replication within alveolar macrophages. Prior to intracellular replication, the vacuole harboring the bacterium must first escape trafficking to the host lysosome, a process that is dependent on the Dot/Icm type IV secretion system. To identify genes required for intracellular growth, bacterial mutants were isolated that were delayed in escape from the macrophage but which retain a minimally functional Dot/Icm machinery. The mutations were found in eight distinct genes, including three genes known to be required for optimal intracellular growth. Two of these genes, icmF and dotU, are located at one end of a cluster of genes that encode the type IV secretion system, yet both icmF and dotU lack orthologs in other type IV translocons. DotU protein is degraded in the early postexponential phase in wild-type L. pneumophila and at all growth phases in an icmF mutant. IcmF contains an extracytoplasmic domain(s) based on accessibility to a membrane-impermeant amine-reactive reagent. In the absence of either gene, L. pneumophila targets inappropriately to LAMP-1-positive compartments during macrophage infection, is defective in the formation of replicative vacuoles, and is impaired in the translocation of the effector protein SidC. Therefore, although IcmF and DotU do not appear to be part of the core type IV secretion system, these proteins are necessary for an efficiently functioning secretion apparatus.
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