ArticleInfection and immunity2004
Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- A subset of type 4 secretion system effectors ofmicroLife · 2026Article
- Biological nanoparticles from Brucella abortusJournal of nanobiotechnology · 2025Article
- Article
- CD150-dependent hematopoietic stem cell sensing of Brucella instructs myeloid commitment.The Journal of experimental medicine · 2023Article
- Comparative proteomic analysis of outer membrane vesicles from Brucella suis, Brucella ovis, Brucella canis and Brucella neotomae.Archives of microbiology · 2021Article
- Proteomics of Brucella.Proteomes · 2020Review
- Outer Membrane Vesicles FromFrontiers in microbiology · 2020Article
- Proteomic Analysis of Membrane Blebs ofFrontiers in microbiology · 2019Article
- Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression.Journal of bacteriology · 2014Article
- Impact of Hfq on global gene expression and intracellular survival in Brucella melitensis.PloS one · 2013Article
- A history of the development of Brucella vaccines.BioMed research international · 2013Review
- Hemin binding protein C is found in outer membrane vesicles and protects Bartonella henselae against toxic concentrations of hemin.Infection and immunity · 2012Article
- Characterization of outer membrane vesicles from Brucella melitensis and protection induced in mice.Clinical & developmental immunology · 2012Article
- Characterization of recombinant B. abortus strain RB51SOD toward understanding the uncorrelated innate and adaptive immune responses induced by RB51SOD compared to its parent vaccine strain RB51.Frontiers in cellular and infection microbiology · 2011Article
- Genomic island 2 of Brucella melitensis is a major virulence determinant: functional analyses of genomic islands.Journal of bacteriology · 2008Article
- BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence.Infection and immunity · 2007Article
- Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis.Infection and immunity · 2007Article
- Mutations of the quorum sensing-dependent regulator VjbR lead to drastic surface modifications in Brucella melitensis.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The survival and replication of Brucella in macrophages is initially triggered by a low intraphagosomal pH. In order to identify proteins released by Brucella during this early acidification step, we analyzed Brucella suis conditioned medium at various pH levels. No significant proteins were released at pH 4.0 in minimal medium or citrate buffer, whereas in acetate buffer, B. suis released a substantial amount of soluble proteins. Comparison of 13 N-terminal amino acid sequences determined by Edman degradation with their corresponding genomic sequences revealed that all of these proteins possessed a signal peptide indicative of their periplasmic location. Ten proteins are putative substrate binding proteins, including a homologue of the nopaline binding protein of Agrobacterium tumefaciens. The absence of this homologue in Brucella melitensis was due to the deletion of a 7.7-kb DNA fragment in its genome. We also characterized for the first time a hypothetical 9.8-kDa basic protein composed of five amino acid repeats. In B. suis, this protein contained 9 repeats, while 12 were present in the B. melitensis orthologue. B. suis in acetate buffer depended on neither the virB type IV secretory system nor the omp31 gene product. However, the integrity of the omp25 gene was required for release at acidic pH, while the absence of omp25b or omp25c displayed smaller effects. Together, these results suggest that Omp25 is involved in the membrane permeability of Brucella in acidic medium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.