ArticleInfection and immunity2004
Functional analysis of the Tsh autotransporter from an avian pathogenic Escherichia coli strain.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Trial
- Spleen Histopathological Evaluation of Broiler Chickens Challenged withVeterinary sciences · 2025Article
- The Role ofVeterinary sciences · 2025Article
- Updates on the Virulence Factors Produced by Multidrug-ResistantMicroorganisms · 2023Review
- Porcine extraintestinal pathogenicFrontiers in cellular and infection microbiology · 2023Article
- Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenicFrontiers in microbiology · 2022Review
- Article
- The Serine Protease Autotransporters TagB, TagC, and Sha from Extraintestinal PathogenicInternational journal of molecular sciences · 2020Article
- Pathogenicity Factors of Genomic Islands in Intestinal and ExtraintestinalFrontiers in microbiology · 2020Review
- Three new serine-protease autotransporters ofVirulence · 2019Article
- Serine Protease Autotransporters of theMicroorganisms · 2019Review
- Type V Secretion Systems: An Overview of Passenger Domain Functions.Frontiers in microbiology · 2019Review
- Not just an antibiotic target: Exploring the role of type I signal peptidase in bacterial virulence.Bioorganic & medicinal chemistry · 2016Article
- Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path.Applied and environmental microbiology · 2016Article
- Subdomain 2 of the Autotransporter Pet Is the Ligand Site for Recognizing the Pet Receptor on the Epithelial Cell Surface.Infection and immunity · 2016Article
- The virulence factor ychO has a pleiotropic action in an Avian Pathogenic Escherichia coli (APEC) strain.BMC microbiology · 2016Article
- TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain.Infection and immunity · 2015Article
- Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence.Cellular and molecular life sciences : CMLS · 2014Review
- Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective.Frontiers in microbiology · 2013Review
- Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins.Applied and environmental microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The temperature-sensitive hemagglutinin (Tsh) is an autotransporter protein secreted by avian-pathogenic Escherichia coli strains that colonize the respiratory tract and lead to airsacculitis, pericarditis, and colisepticemia. It is synthesized as a 140-kDa precursor protein, whose processing results in a 106-kDa passenger domain (Tshs) and a 33-kDa beta-domain (Tsh(beta)). The presence of a conserved 7-amino-acid serine protease motif within Tshs classifies the protein in a subfamily of autotransporters, known as serine protease autotransporters of the Enterobacteriaceae. In this study, we report that purified Tshs is capable of adhering to red blood cells, hemoglobin, and the extracellular matrix proteins fibronectin and collagen IV. We also demonstrate that Tshs exerts proteolytic activity against casein, and we provide experimental evidence demonstrating that serine 259 is essential for the protease function. However, this residue is not required for adherence to substrates, and its replacement by an alanine does not abolish binding activity. In summary, our results demonstrate that Tsh is a bifunctional protein with both adhesive and proteolytic properties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.