ArticleRNA (New York, N.Y.)2004
Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Liquid-liquid phase separation and the formation of amyloid fibrils from DcpS scavenger enzymes.Scientific reports · 2026Article
- Programmed DNA elimination in the parasitic nematode Ascaris.PLoS pathogens · 2023Review
- Eukaryotic mRNA Decapping Activation.Frontiers in genetics · 2022Review
- Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing.Nucleic acids research · 2021Article
- Genomics of the Parasitic NematodeGenes · 2021Review
- Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs.ACS omega · 2020Article
- Decapping Scavenger Enzyme Activity toward N2-Substituted 5' End mRNA Cap Analogues.ACS omega · 2019Article
- The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping.Scientific reports · 2019Article
- mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation.Nucleic acids research · 2017Article
- Nuclei Isolation from NematodeBio-protocol · 2017Article
- The human decapping scavenger enzyme DcpS modulates microRNA turnover.Scientific reports · 2015Article
- Elimination of cap structures generated by mRNA decay involves the new scavenger mRNA decapping enzyme Aph1/FHIT together with DcpS.Nucleic acids research · 2015Article
- Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.Nucleic acids research · 2014Article
- The decapping scavenger enzyme DCS-1 controls microRNA levels in Caenorhabditis elegans.Molecular cell · 2013Article
- Identification of Drosophila and human 7-methyl GMP-specific nucleotidases.The Journal of biological chemistry · 2013Article
- 7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity.Biochemistry · 2012Article
- Synthesis of N²-modified 7-methylguanosine 5'-monophosphates as nematode translation inhibitors.Bioorganic & medicinal chemistry · 2012Article
- Synthesis of ¹³C- and ¹⁴C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies.Bioorganic & medicinal chemistry letters · 2012Article
- Affinity resins containing enzymatically resistant mRNA cap analogs--a new tool for the analysis of cap-binding proteins.RNA (New York, N.Y.) · 2012Article
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A spliced leader contributes the mature 5'ends of many mRNAs in trans-splicing organisms. Trans-spliced metazoan mRNAs acquire an m3(2,2,7)GpppN cap from the added spliced leader exon. The presence of these caps, along with the typical m7GpppN cap on non-trans-spliced mRNAs, requires that cellular mRNA cap-binding proteins and mRNA metabolism deal with different cap structures. We have developed and used an in vitro system to examine mRNA degradation and decapping activities in nematode embryo extracts. The predominant pathway of mRNA decay is a 3' to 5' pathway with exoribonuclease degradation of the RNA followed by hydrolysis of resulting mRNA cap by a scavenger (DcpS-like) decapping activity. Direct decapping of mRNA by a Dcp1/Dcp2-like activity does occur, but is approximately 15-fold less active than the 3' to 5' pathway. The DcpS-like activity in nematode embryo extracts hydrolyzes both m7GpppG and m3(2,2,7)GpppG dinucleoside triphosphates. The Dcp1/Dcp2-like activity in extracts also hydrolyzes these two cap structures at the 5' ends of RNAs. Interestingly, recombinant nematode DcpS differs from its human ortholog in its substrate length requirement and in its capacity to hydrolyze m3(2,2,7)GpppG.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.