ArticleJournal of anatomy2004
Hyaline cartilage engineered by chondrocytes in pellet culture: histological, immunohistochemical and ultrastructural analysis in comparison with cartilage explants.
Article in Journal of anatomy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 139 citations in OpenAlex.
- Supramolecular Engineering of Fluid Pressure in Filamentous Hybrid Double Network Hydrogels for 3D Chondrocyte Culture.Advanced healthcare materials · 2026Article
- Understanding the use of spheroids and pellets in the chondrogenic differentiation of human stem cells.Osteoarthritis and cartilage open · 2026Article
- Expansion and Delivery of Human Chondrocytes on Gelatin-Based Cell Carriers.Gels (Basel, Switzerland) · 2025Article
- Recent advancements in cartilage tissue engineering innovation and translation.Nature reviews. Rheumatology · 2024Review
- Irisin Role in Chondrocyte 3D Culture Differentiation and Its Possible Applications.Pharmaceutics · 2023Article
- Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering.Journal of functional biomaterials · 2022Article
- A humanOsteoarthritis and cartilage open · 2022Article
- Insights into the present and future of cartilage regeneration and joint repair.Cell regeneration (London, England) · 2022Review
- In Vitro Study of Extracellular Vesicles Migration in Cartilage-Derived Osteoarthritis Samples Using Real-Time Quantitative Multimodal Nonlinear Optics Imaging.Pharmaceutics · 2020Article
- GDF5 induces TBX3 in a concentration dependent manner - on a gold nanoparticle gradient.Heliyon · 2020Article
- Chondrogenic Potential of Pellet Culture Compared to High-Density Culture on a Bacterial Cellulose Hydrogel.International journal of molecular sciences · 2020Article
- Collagen microsphere based 3D culture system for human osteoarthritis chondrocytes (hOACs).Scientific reports · 2019Article
- An Innovative Laboratory Procedure to Expand Chondrocytes with Reduced Dedifferentiation.Cartilage · 2018Article
- Chondrogenic Potential of Dedifferentiated Rat Chondrocytes Reevaluated in Two- and Three-Dimensional Culture Conditions.Tissue engineering and regenerative medicine · 2018Article
- Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration.Stem cells international · 2018Review
- Down-Regulation of Transglutaminase 2 Stimulates Redifferentiation of Dedifferentiated Chondrocytes through Enhancing Glucose Metabolism.International journal of molecular sciences · 2017Article
- Near infrared spectroscopic assessment of developing engineered tissues: correlations with compositional and mechanical properties.The Analyst · 2017Article
- Poly(γ-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.Tissue engineering. Part A · 2015Article
- Harnessing biomechanics to develop cartilage regeneration strategies.Journal of biomechanical engineering · 2015Review
- Mechanical characterization of tissue-engineered cartilage using microscopic magnetic resonance elastography.Tissue engineering. Part C, Methods · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cartilage engineering is a strategic experimental goal for the treatment of multiple joint diseases. Based on the process of embryonic chondrogenesis, we hypothesized that cartilage could be engineered by condensing chondrocytes in pellet culture and, in the present study, examined the quality of regenerated cartilage in direct comparison with native cartilage. Chondrocytes isolated from the sterna of chick embryos were cultured in pellets (4 x 10(6) cells per pellet) for 2 weeks. Cartilage explants from the same source were cultured as controls. After 2 weeks, the regenerated cartilage from pellet culture had a disc shape and was on average 9 mm at the longest diameter. The chondrocyte phenotype was stabilized in pellet culture as shown by the synthesis of type II collagen and aggrecan, which was the same intensity as in the explant after 7 days in culture. During culture, chondrocytes also continuously synthesized type IX collagen. Type X collagen was negatively stained in both pellets and explants. Except for fibril orientation, collagen fibril diameter and density in the engineered cartilage were comparable with the native cartilage. In conclusion, hyaline cartilage engineered by chondrocytes in pellet culture, without the transformation of cell phenotypes and scaffold materials, shares similarities with native cartilage in cellular distribution, matrix composition and density, and ultrastructure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.