ArticleBiochemistry2004
Fetal Alz-50 clone 1 interacts with the human orthologue of the Kelch-like Ech-associated protein.
Article in Biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- A prelude to the proximity interaction mapping of CXXC5.Scientific reports · 2021Article
- Opportunity knocks for uncovering the new function of an understudied nucleosome remodeling complex member, the bromodomain PHD finger transcription factor, BPTF.Current opinion in chemical biology · 2021Review
- Random forest-integrated analysis in AD and LATE brain transcriptome-wide data to identify disease-specific gene expression.PloS one · 2021Article
- Beyond repression of Nrf2: An update on Keap1.Free radical biology & medicine · 2020Review
- Article
- NRP2 transcriptionally regulates its downstream effector WDFY1.Scientific reports · 2016Article
- Binding of disordered proteins to a protein hub.Scientific reports · 2013Article
- Comparison of Secondary Structure Formation Using 10 Different Force Fields in Microsecond Molecular Dynamics Simulations.Journal of chemical theory and computation · 2012Article
- Inhibitor of Nrf2 (INrf2 or Keap1) protein degrades Bcl-xL via phosphoglycerate mutase 5 and controls cellular apoptosis.The Journal of biological chemistry · 2011Article
- The nucleosome remodeling factor.FEBS letters · 2011Review
- INrf2 (Keap1) targets Bcl-2 degradation and controls cellular apoptosis.Cell death and differentiation · 2011Article
- Stress-activated cap'n'collar transcription factors in aging and human disease.Science signaling · 2010Review
- PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria.Experimental cell research · 2008Article
- Focal adhesions regulate Abeta signaling and cell death in Alzheimer's disease.Biochimica et biophysica acta · 2007Review
- Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The fetal Alz-50 reactive clone 1 (FAC1) protein exhibits altered expression and subcellular localization during neuronal development and neurodegenerative diseases such as Alzheimer's disease. Using the yeast two-hybrid screen, the human orthologue of Keap1 (hKeap1) was identified as a FAC1 interacting protein. Keap1 is an important regulator of the oxidative stress response pathway through its interaction with the Nrf family of transcription factors. An interaction between full-length FAC1 and hKeap1 proteins has been demonstrated, and the FAC1 binding domain of hKeap1 has been identified as the Kelch repeats. In addition, FAC1 colocalizes with endogenous Keap1 within the cytoplasm of PT67 cells. Exogenously introduced eGFP:hKeap1 fusion protein redistributed FAC1 to colocalize with eGFP:hKeap1 in perinuclear, spherical structures. The interaction between FAC1 and hKeap1 is reduced by competition with the Nrf2 protein. However, competition by Nrf2 for hKeap1 is reduced by diethylmaleate (DEM), a known disrupter of the Nrf2:Keap1 interaction. DEM does not affect the ability of FAC1 to bind hKeap1 in our assay. These results suggest that hKeap1 regulates FAC1 in addition to its known role in control of Nrf2. Furthermore, the observed competition between FAC1 and Nrf2 for binding hKeap1 indicates that the interplay between these three proteins has important implications for neuronal response to oxidative stress.
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