ArticleJournal of clinical microbiology1992
Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard?
Article in Journal of clinical microbiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 169 citations in OpenAlex.
- Inter- and intra-test agreement of three commercially available molecular diagnostic tests for the identification of periodontal pathogens.Clinical oral investigations · 2015Trial
- Bacterial genera in the fluids from apical periodontitis-related radicular cysts: An observational study.International endodontic journal · 2025Observational
- A magnetic nanozyme platform for bacterial colorimetric detection and chemodynamic/photothermal synergistic antibacterial therapy.Mikrochimica acta · 2024Article
- Portable biosensor-based oral pathogenic bacteria detection for community and family applications.Analytical and bioanalytical chemistry · 2023Review
- Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide.Clinical and experimental dental research · 2021Article
- Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens.Clinical oral investigations · 2016Article
- Culture-based identification of pigmented Porphyromonas and Prevotella species in primary endodontic infections.Journal of dental research, dental clinics, dental prospects · 2016Article
- Relationship between gingival bleeding and anaerobic periodontal infection assessed by BANA (N-Benzoyl-DL-Arginine-β-Napthylamide) assay.Journal of pharmacy & bioallied sciences · 2014Article
- Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results.European journal of dentistry · 2013Article
- Efficacy of Subgingival Application of Xanthan-based Chlorhexidine Gel Adjunctive to Full-mouth Root Planing Assessed by Real-time PCR: A Microbiologic and Clinical Study.Journal of dental research, dental clinics, dental prospects · 2013Article
- Evaluation of two mouth rinses in reduction of oral malodor using a spectrophotometric technique.Journal of Indian Society of Periodontology · 2011Article
- Evaluation of Genetic Pattern of Gentamicin-resistant Enterococci Isolated from Clinical Samples.Journal of dental research, dental clinics, dental prospects · 2011Article
- Adaptation of Porphyromonas gingivalis to microaerophilic conditions involves increased consumption of formate and reduced utilization of lactate.Microbiology (Reading, England) · 2009Article
- Population-based study of salivary carriage of periodontal pathogens in adults.Journal of clinical microbiology · 2007Article
- Article
- Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment.Clinical microbiology reviews · 2001Review
- Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR.Journal of clinical microbiology · 1999Article
- Proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria.Journal of clinical microbiology · 1999Article
- Isolation of Porphyromonas gingivalis and detection of immunoglobulin A specific to fimbrial antigen in gingival crevicular fluid.Journal of clinical microbiology · 1998Article
- Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.Journal of clinical microbiology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The development of diagnostic tests for a periodontal infection raises the issue as to what the appropriate reference standard, or "gold standard," should be for the evaluation of a new test. The present research was initiated to compare the ability of several detection methods, i.e., a serial dilution anaerobic culture and/or microscopic procedure, a DNA probe procedure, and immunological reagents using both an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay to detect Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus, and Actinobacillus actinomycetemcomitans in subgingival plaque samples taken from 204 periodontally diseased tooth sites. The prevalence of the four monitored species varied as a function of both the species and the detection method. Spirochetes were present in 99% of the plaques, whereas A. actinomycetemcomitans was detected at the lowest frequency. The culture method yielded the lowest prevalence values for the three cultivable species. This raised the question as to which results, those obtained by culture or those obtained by the DNA probes and the immunological reagents, were the most reliable. This issue was addressed by looking at the prevalence profile of the monitored organisms, as determined by all the detection methods. If the species was detected by three or four of the detection methods, then it was considered present, whereas if it was absent by three or four of the detection methods, then it was considered absent. This approach showed the DNA probes and immunological reagents to be significantly superior (P less than 0.05) to the culture approach for the detection of P. gingivalis, A. actinomycetemcomitans, and B. forsythus and to be comparable to the microscopic approach in the detection of T. denticola.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.