ArticleWorld journal of gastroenterology2004
siRNA-mediated inhibition of HBV replication and expression.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Cell Penetrating Peptides Used in Delivery of Therapeutic Oligonucleotides Targeting Hepatitis B Virus.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Progress in the use of RNA interference as a therapy for chronic hepatitis B virus infection.Genome medicine · 2010Article
- Hepatitis B and hepatitis C virus replication upregulates serine protease inhibitor Kazal, resulting in cellular resistance to serine protease-dependent apoptosis.Journal of virology · 2010Article
- Hepatitis B virus (HBV)-specific short hairpin RNA is capable of reducing the formation of HBV covalently closed circular (CCC) DNA but has no effect on established CCC DNA in vitro.The Journal of general virology · 2009Article
- Vector design for liver-specific expression of multiple interfering RNAs that target hepatitis B virus transcripts.Antiviral research · 2008Article
- Combination of small interfering RNAs mediates greater suppression on hepatitis B virus cccDNA in HepG2.2.15 cells.World journal of gastroenterology · 2008Article
- Targeted screening of SiRNA directed HBV polymerase gene for effective inhibition of HBV expression.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2008Article
- Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells.World journal of gastroenterology · 2007Article
- Inhibition of hepatitis B virus expression and replication by RNA interference in HepG2.2.15.World journal of gastroenterology · 2006Article
- Review
- Inhibition of viruses by RNA interference.Virus genes · 2006Review
- Cationized gelatin delivery of a plasmid DNA expressing small interference RNA for VEGF inhibits murine squamous cell carcinoma.Cancer science · 2006Article
- Therapeutic short hairpin RNA expression in the liver: viral targets and vectors.Gene therapy · 2006Review
- Criteria for effective design, construction, and gene knockdown by shRNA vectors.BMC biotechnology · 2006Article
- RNA interference: its use as antiviral therapy.Handbook of experimental pharmacology · 2006Review
- New therapeutics targets in chronic viral cardiomyopathy.Ernst Schering Research Foundation workshop · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimRNA interference (RNAi) is a newly discovered phenomenon provoked by dsRNA. The dsRNA is initially cleaved by Dicer into 21-23 nt small interfering RNA (siRNA) and can then specifically target homologous mRNA for degradation by cellular ribonucleases. RNAi has been successfully utilized to down-regulate the endogenous gene expression or suppress the replication of various pathogens in mammalian cells. In this study, we investigated whether vector-based siRNA promoted by U6 (pSilencer1.0-U6) could efficiently inhibit HBV replication in cell culture.
methodspSilencer vectors with inserts targeting on different regions of HBV genome were constructed. These plasmids were co-transfected with pHBV3.8 into Huh-7 cells via lipofection and viral antigens were measured by ELISA. Viral RNA was analyzed by Northern blot. The mRNA of MxA and 2'-5'OAS was reverse transcribed and quantified by real-time PCR.
resultsVector-based siRNA could potently reduce hepatitis B virus antigen expression in transient replicative cell culture. Furthermore, Northern blot analysis showed that viral RNA was effectively degraded, thus eliminating the messengers for protein expression as well as template for reverse transcription. Real-time PCR analysis of cellular MxA and 2'-5'OAS gene expression revealed that vector-based siRNA did not provoke the interferon pathway which reassured the specificity of the vector-based RNA interference technique.
conclusionOur results indicate that RNA interference may be a potential tool to control HBV infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.