ArticleBiophysical journal2004
Imaging native beta-actin mRNA in motile fibroblasts.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 148 citations in OpenAlex.
- Subcellular localization as a driver of protein function.Nature reviews. Molecular cell biology · 2026Review
- Live-Cell Imaging of Multiple Endogenous mRNAs Using RNA Aptamers and Chemical Probes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA.iScience · 2024Article
- Uncovering Molecular Quencher Effects on FRET Phenomena in Microsphere-Immobilized Probe Systems.Analytical chemistry · 2023Article
- Progress of CRISPR-Cas13 Mediated Live-Cell RNA Imaging and Detection of RNA-Protein Interactions.Frontiers in cell and developmental biology · 2022Review
- Advances of Exosomal miRNAs in Breast Cancer Progression and Diagnosis.Diagnostics (Basel, Switzerland) · 2021Review
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.eLife · 2019Article
- β-Actin mRNA interactome mapping by proximity biotinylation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.Nucleic acids research · 2018Article
- Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization.Genomics, proteomics & bioinformatics · 2017Review
- A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.Scientific reports · 2017Article
- Nucleic Acid-Based Nanodevices in Biological Imaging.Annual review of biochemistry · 2016Review
- Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells.Methods (San Diego, Calif.) · 2016Review
- RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.Current protocols in chemical biology · 2016Article
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Live-cell imaging of mammalian RNAs with Spinach2.Methods in enzymology · 2015Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nuclease-resistant, cytoplasmically resident molecular beacons were used to specifically label beta-actin mRNA in living and motile chicken embryonic fibroblasts. beta-actin mRNA signals were most abundant in active lamellipodia, which are protrusions that cells extend to adhere to surfaces. Time-lapse images show that the immediate sources of beta-actin mRNA for nascent lamellipodia are adjacent older protrusions. During the development of this method, we observed that conventional molecular beacons are rapidly sequestered in cell nuclei, leaving little time for them to find and bind to their cytoplasmic mRNA targets. By linking molecular beacons to a protein that tends to stay within the cytoplasm, nuclear sequestration was prevented, enabling cytoplasmic mRNAs to be detected and imaged. Probing beta-actin mRNA with these cytoplasmically resident molecular beacons did not affect the motility of the fibroblasts. Furthermore, mRNAs bound to these probes undergo translation within the cell. The use of cytoplasmically resident molecular beacons will enable further studies of the mechanism of beta-actin mRNA localization, and will be useful for understanding the dynamics of mRNA distribution in other living cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.