ArticleImmunology1992
Production of granulocyte-macrophage colony-stimulating factor by cultured human tracheal epithelial cells.
Article in Immunology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 105 citations in OpenAlex.
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- Granulocyte-Macrophage Colony-Stimulating Factor as a Therapeutic Target in Multiple Sclerosis.Neurology and therapy · 2019Review
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- Chemokine transcripts as targets of the RNA-binding protein HuR in human airway epithelium.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Role of the RNA-binding protein tristetraprolin in glucocorticoid-mediated gene regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Induction of B7-H1 and B7-DC expression on airway epithelial cells by the Toll-like receptor 3 agonist double-stranded RNA and human rhinovirus infection: In vivo and in vitro studies.The Journal of allergy and clinical immunology · 2008Article
- Versatile applications of transcriptional pulsing to study mRNA turnover in mammalian cells.RNA (New York, N.Y.) · 2007Article
- Time course of gene expression of inflammatory mediators in rat lung after diesel exhaust particle exposure.Environmental health perspectives · 2005Article
- The sources of inflammatory mediators in the lung after silica exposure.Environmental health perspectives · 2004Article
- Induction of tumor necrosis factor alpha and interleukin-8 gene expression in bronchial epithelial cells by toxic shock syndrome toxin 1.Infection and immunity · 2000Article
- GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice.The Journal of clinical investigation · 1998Article
- Expression of eotaxin by human lung epithelial cells: induction by cytokines and inhibition by glucocorticoids.The Journal of clinical investigation · 1997Article
- Pulmonary epithelial cell expression of GM-CSF corrects the alveolar proteinosis in GM-CSF-deficient mice.The Journal of clinical investigation · 1996Article
- Nasal hyperreactivity and inflammation in allergic rhinitis.Mediators of inflammation · 1996Article
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- Inhibition of the immunosuppressive activity of resident pulmonary alveolar macrophages by granulocyte/macrophage colony-stimulating factor.The Journal of experimental medicine · 1993Article
- An overview of glucocorticoid anti-inflammatory actions.European journal of clinical pharmacology · 1993Review
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have evaluated the capacity of cultured human respiratory epithelial cells (HTE) to produce granulocyte-macrophage colony-stimulating factor (GM-CSF) and have examined the ability of proinflammatory stimuli and of glucocorticoids to modulate the production of GM-CSF by these cells. Conditioned medium (CM) was obtained after 24-hr culture of HTE in the presence or absence of serum (5%) and was assayed for GM-CSF activity using the M-07e cell line, which proliferates in response to GM-CSF and interleukin-3 (IL-3). HTE produced 1.1 +/- 0.7 and 2.1 +/- 1.5 ng GM-CSF/10(6) cells in the presence or absence of serum, respectively (n = 4). The identity of this activity as GM-CSF was established by neutralization with specific antibody to GM-CSF, while antibody to IL-3 was without effect. Dexamethasone (10(-6) M) inhibited basal GM-CSF release to below the limit of detection of the assay (0.12 ng GM-CSF/ml conditioned media). GM-CSF release was significantly enhanced by 10(-6) M histamine (1.6 +/- 0.7 versus 2.13 +/- 0.8 ng GM-CSF/10(6) cells; n = 9) and 5 ng/ml interleukin-1 (IL-1) (0.6 +/- 0.2 versus 3.2 +/- 0.5 ng GM-CSF/10(6) cells; n = 3). Stimulation of GM-CSF release by IL-1 was dose-dependent. A significant increase in GM-CSF activity was observed with 0.1 ng/ml, while maximal stimulation occurred at 5 ng/ml IL-1. In kinetic studies, GM-CSF activity was first detected in CM from cells incubated in the absence of stimulus at 8 hr of incubation, and continued to increase up to 24 hr. IL-1 stimulated GM-CSF activity was detected in CM as early as 4 hr and continued to increase significantly up to 24 hr. Thus, human tracheal epithelial cells release GM-CSF and this release is regulated by inflammatory mediators and glucocorticoids.
Indexed as
Identifiers
1537596PMC1384823W2468470270What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.