ArticlePlant cell reports2005
Overexpression of the feedback-insensitive anthranilate synthase gene in tobacco causes tryptophan accumulation.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- Genetic engineering to improve essential and conditionally essential amino acids in maize: transporter engineering as a reference.Transgenic research · 2021Article
- Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco.Plant physiology · 2015Article
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection.Plant cell reports · 2008Article
- Use of 4-methylindole or 7-methyl-DL-tryptophan in a transformant selection system based on the feedback-insensitive anthranilate synthase alpha-subunit of tobacco (ASA2).Plant cell reports · 2008Article
- Characterization of tryptamine 5-hydroxylase and serotonin synthesis in rice plants.Plant cell reports · 2007Article
- Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.Plant cell reports · 2007Article
- The genetic manipulation of medicinal and aromatic plants.Plant cell reports · 2007Review
- Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation.Planta · 2007Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 35 independent transgenic tobacco plants were produced using the Agrobacterium tumefaciens-leaf segment co-cultivation method followed by selection with kanamycin for the nptII gene. The vector also carried the tobacco feedback-insensitive anthranilate synthase gene (ASA2). Many of the lines showed increased ASA2 mRNA levels but only three contained increased free tryptophan (Trp) and many lines contained lower Trp than the untransformed control. The line with the highest Trp level (threefold that of the untransformed control) contained increased anthranilate synthase activity (AS) both in leaves and a cell suspension culture derived from the plant while the feedback insensitivity was most evident in the suspension culture. Other kinetic data also indicated that the ASA2 encoded AS alpha-subunit was more abundant in the tissue culture than in leaves. Progeny seedlings from this line were resistant to certain toxic Trp analogs, especially alpha-methyltryptophan (alphaMT) and less so to the most commonly used analog, 5-methyltryptophan. Shoots formed more readily from leaves of two of the transgenic lines than from leaves of the untransformed control on alphaMT, indicating that it might be possible to use ASA2 as a selectable marker gene and alphaMT as the selection agent.
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