ArticleThe Journal of biological chemistry2004
Endonuclease-mediated mRNA decay requires tyrosine phosphorylation of polysomal ribonuclease 1 (PMR1) for the targeting and degradation of polyribosome-bound substrate mRNA.
Article in The Journal of biological chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- RNA Metabolism in T Lymphocytes.Immune network · 2022Review
- RNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machinery.Nucleic acids research · 2016Article
- Temporal dynamics of the Saccharopolyspora erythraea phosphoproteome.Molecular & cellular proteomics : MCP · 2014Article
- SMG6 cleavage generates metastable decay intermediates from nonsense-containing β-globin mRNA.PloS one · 2013Article
- The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.PLoS pathogens · 2013Article
- Identification of the human PMR1 mRNA endonuclease as an alternatively processed product of the gene for peroxidasin-like protein.RNA (New York, N.Y.) · 2012Article
- Review
- Regulation of cytoplasmic mRNA decay.Nature reviews. Genetics · 2012Review
- Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.RNA (New York, N.Y.) · 2010Review
- KSRP-PMR1-exosome association determines parathyroid hormone mRNA levels and stability in transfected cells.BMC cell biology · 2009Article
- The cytoskeleton-associated Ena/VASP proteins are unanticipated partners of the PMR1 mRNA endonuclease.RNA (New York, N.Y.) · 2009Article
- Decoding ARE-mediated decay: is microRNA part of the equation?The Journal of cell biology · 2008Review
- The 90-kDa heat shock protein stabilizes the polysomal ribonuclease 1 mRNA endonuclease to degradation by the 26S proteasome.Molecular biology of the cell · 2008Article
- Approaches for studying PMR1 endonuclease-mediated mRNA decay.Methods in enzymology · 2008Article
- c-Src activates endonuclease-mediated mRNA decay.Molecular cell · 2007Article
- Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1.Molecular and cellular biology · 2006Article
- The poly(A)-limiting element enhances mRNA accumulation by increasing the efficiency of pre-mRNA 3' processing.RNA (New York, N.Y.) · 2005Article
- Mechanisms of endonuclease-mediated mRNA decay.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
PMR1 is an endonuclease that is activated by estrogen to degrade Xenopus albumin mRNA. A previous report showed that the functional unit of endonuclease-mediated mRNA decay is a approximately 680-kDa polysome-bound complex that contains both PMR1 and substrate mRNA. PMR1 contains two domains involved in endonuclease targeting to polysomes, an N-terminal domain that lies between residues 200 and 250, and a C-terminal domain that lies within the last 100 residues. Loss of either domain inactivated PMR1 targeting to polysomes and stabilized albumin mRNA. The current study identified a phosphorylated tyrosine residue within the C-terminal polysome-targeting domain and showed that this modification is required for PMR1-mediated mRNA decay. Changing this tyrosine to phenylalanine inactivated the targeting of PMR1 to polysomes, blocked binding of PMR1 to the functional complex containing its substrate mRNA, prevented the targeting of a green fluorescent protein fusion protein to this complex, and stabilized albumin mRNA to degradation by PMR1 in vivo. A general tyrosine kinase inhibitor inhibited the phosphorylation of PMR1, which in turn inhibited PMR1-catalyzed degradation of albumin mRNA. These results indicate that one or more tyrosine kinases functions as a regulator of endonuclease-mediated mRNA decay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.