ArticleJournal of bacteriology2004
Involvement of exo5 in production of surface polysaccharides in Rhizobium leguminosarum and its role in nodulation of Vicia sativa subsp. nigra.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Black Carbon Impacts onEnvironmental science & technology · 2024Article
- Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes.International journal of molecular sciences · 2021Review
- Mutation in theGenes · 2018Article
- Conserved Composition of Nod Factors and Exopolysaccharides Produced by Different Phylogenetic LineageFrontiers in microbiology · 2018Article
- The Rhizobia-Frontiers in microbiology · 2018Review
- Gene expression and localization of a β-1,3-glucanase of Lotus japonicus.Journal of plant research · 2016Article
- How legumes recognize rhizobia.Plant signaling & behavior · 2016Review
- AFrontiers in microbiology · 2016Article
- The Regulatory Protein RosR Affects Rhizobium leguminosarum bv. trifolii Protein Profiles, Cell Surface Properties, and Symbiosis with Clover.Frontiers in microbiology · 2016Article
- Relevance of fucose-rich extracellular polysaccharides produced by Rhizobium sullae strains nodulating Hedysarum coronarium l. legumes.Applied and environmental microbiology · 2013Article
- RapA2 is a calcium-binding lectin composed of two highly conserved cadherin-like domains that specifically recognize Rhizobium leguminosarum acidic exopolysaccharides.The Journal of biological chemistry · 2013Article
- Article
- Optimization of exopolysaccharide production and diesel oil emulsifying properties in root nodulating bacteria.World journal of microbiology & biotechnology · 2012Article
- Environmental signals and regulatory pathways that influence exopolysaccharide production in rhizobia.International journal of molecular sciences · 2011Review
- Biosynthesis of UDP-xylose and UDP-arabinose in Sinorhizobium meliloti 1021: first characterization of a bacterial UDP-xylose synthase, and UDP-xylose 4-epimerase.Microbiology (Reading, England) · 2011Article
- Structures of the lipopolysaccharides from Rhizobium leguminosarum RBL5523 and its UDP-glucose dehydrogenase mutant (exo5).Glycobiology · 2011Article
- The novel genes emmABC are associated with exopolysaccharide production, motility, stress adaptation, and symbiosis in Sinorhizobium meliloti.Journal of bacteriology · 2009Article
- Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula.Plant physiology · 2008Article
- LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.The EMBO journal · 2007Article
- Expression cloning of three Rhizobium leguminosarum lipopolysaccharide core galacturonosyltransferases.The Journal of biological chemistry · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of two exopolysaccharide-deficient mutants of Rhizobium leguminosarum, RBL5808 and RBL5812, revealed independent Tn5 transposon integrations in a single gene, designated exo5. As judged from structural and functional homology, this gene encodes a UDP-glucose dehydrogenase responsible for the oxidation of UDP-glucose to UDP-glucuronic acid. A mutation in exo5 affects all glucuronic acid-containing polysaccharides and, consequently, all galacturonic acid-containing polysaccharides. Exo5-deficient rhizobia do not produce extracellular polysaccharide (EPS) or capsular polysaccharide (CPS), both of which contain glucuronic acid. Carbohydrate composition analysis and nuclear magnetic resonance studies demonstrated that EPS and CPS from the parent strain have very similar structures. Lipopolysaccharide (LPS) molecules produced by the mutant strains are deficient in galacturonic acid, which is normally present in the core and lipid A portions of the LPS. The sensitivity of exo5 mutant rhizobia to hydrophobic compounds shows the involvement of the galacturonic acid residues in the outer membrane structure. Nodulation studies with Vicia sativa subsp. nigra showed that exo5 mutant rhizobia are impaired in successful infection thread colonization. This is caused by strong agglutination of EPS-deficient bacteria in the root hair curl. Root infection could be restored by simultaneous inoculation with a Nod factor-defective strain which retained the ability to produce EPS and CPS. However, in this case colonization of the nodule tissue was impaired.
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