ArticleJournal of bacteriology2004
Bifidobacterium longum requires a fructokinase (Frk; ATP:D-fructose 6-phosphotransferase, EC 2.7.1.4) for fructose catabolism.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description ofMicroorganisms · 2024Article
- Regulation of Fructose Metabolism in Nonalcoholic Fatty Liver Disease.Biomolecules · 2024Review
- Deciphering the role of recurrent FAD-dependent enzymes in bacterial phosphonate catabolism.iScience · 2023Article
- The Microbial Degradation of Natural and Anthropogenic Phosphonates.Molecules (Basel, Switzerland) · 2023Review
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications inMicroorganisms · 2023Article
- Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum.Nature chemical biology · 2023Article
- Proteomic Signatures of Microbial Adaptation to the Highest Ultraviolet-Irradiation on Earth: Lessons From a Soil Actinobacterium.Frontiers in microbiology · 2022Article
- Carbohydrate-controlled serine protease inhibitor (serpin) production in Bifidobacterium longum subsp. longum.Scientific reports · 2021Article
- The genes and enzymes of sucrose metabolism in moderately thermophilic methanotroph Methylocaldum szegediense O12.Extremophiles : life under extreme conditions · 2018Article
- Molecular and biochemical characterization of Entamoeba histolytica fructokinase.Parasitology research · 2015Article
- The mannitol utilization system of the marine bacterium Zobellia galactanivorans.Applied and environmental microbiology · 2015Article
- Fructose uptake in Bifidobacterium longum NCC2705 is mediated by an ATP-binding cassette transporter.The Journal of biological chemistry · 2012Article
- Mannheimia succiniciproducens phosphotransferase system for sucrose utilization.Applied and environmental microbiology · 2010Article
- A proteome reference map of Trichomonas vaginalis.Parasitology research · 2009Article
- Kinetic analysis of bifidobacterial metabolism reveals a minor role for succinic acid in the regeneration of NAD+ through its growth-associated production.Applied and environmental microbiology · 2006Article
- Lactose-over-glucose preference in Bifidobacterium longum NCC2705: glcP, encoding a glucose transporter, is subject to lactose repression.Journal of bacteriology · 2006Article
- Milk glycan metabolism by intestinal bifidobacteria: insights from comparative genomics.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the ability of Bifidobacterium spp. to grow on fructose as a unique carbon source has been demonstrated, the enzyme(s) needed to incorporate fructose into a catabolic pathway has hitherto not been defined. This work demonstrates that intracellular fructose is metabolized via the fructose-6-P phosphoketolase pathway and suggests that a fructokinase (Frk; EC 2.7.1.4) is the enzyme that is necessary and sufficient for the assimilation of fructose into this catabolic route in Bifidobacterium longum. The B. longum A10C fructokinase-encoding gene (frk) was expressed in Escherichia coli from a pET28 vector with an attached N-terminal histidine tag. The expressed enzyme was purified by affinity chromatography on a Co(2+)-based column, and the pH and temperature optima were determined. A biochemical analysis revealed that Frk displays the same affinity for fructose and ATP (Km(fructose) = 0.739 +/- 0.18 mM and Km(ATP) = 0.756 +/- 0.08 mM), is highly specific for D-fructose, and is inhibited by an excess of ATP (>12 mM). It was also found that frk is inducible by fructose and is subject to glucose-mediated repression. Consequently, this work presents the first characterization at the molecular and biochemical level of a fructokinase from a gram-positive bacterium that is highly specific for D-fructose.
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