ArticleJournal of bacteriology2004
patS minigenes inhibit heterocyst development of Anabaena sp. strain PCC 7120.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Inactivation of Three RG(S/T)GR Pentapeptide-Containing Negative Regulators of HetR Results in Lethal Differentiation ofLife (Basel, Switzerland) · 2020Article
- HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacteriumeLife · 2020Article
- Phenotypic Assessment Suggests Multiple Start Codons for HetN, an Inhibitor of Heterocyst Differentiation, in Anabaena sp. Strain PCC 7120.Journal of bacteriology · 2018Article
- Robust stochastic Turing patterns in the development of a one-dimensional cyanobacterial organism.PLoS biology · 2018Article
- Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium.FEMS microbiology letters · 2017Article
- An amidase is required for proper intercellular communication in the filamentous cyanobacteriumProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- ABC Transporter Required for Intercellular Transfer of Developmental Signals in a Heterocystous Cyanobacterium.Journal of bacteriology · 2015Article
- An integrative approach for modeling and simulation of heterocyst pattern formation in cyanobacteria filaments.PLoS computational biology · 2015Article
- Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Global transcription profiles of the nitrogen stress response resulting in heterocyst or hormogonium development in Nostoc punctiforme.Journal of bacteriology · 2011Article
- Mutations in genes patA and patL of Anabaena sp. strain PCC 7120 result in similar phenotypes, and the proteins encoded by those genes may interact.Journal of bacteriology · 2011Article
- Overexpression of pknE blocks heterocyst development in Anabaena sp. strain PCC 7120.Journal of bacteriology · 2011Article
- Genome erosion in a nitrogen-fixing vertically transmitted endosymbiotic multicellular cyanobacterium.PloS one · 2010Article
- Cyanobacterial heterocysts.Cold Spring Harbor perspectives in biology · 2010Review
- Compartmentalized function through cell differentiation in filamentous cyanobacteria.Nature reviews. Microbiology · 2010Review
- Genetic and cytological evidence that heterocyst patterning is regulated by inhibitor gradients that promote activator decay.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Regions of the amino terminus of the P2X receptor required for modification by phorbol ester and mGluR1alpha receptors.Journal of neurochemistry · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode PatS C-terminal 4 (GSGR) to 8 (CDERGSGR) oligopeptides. When expressed by P(petE), P(patS), or P(rbcL) promoters, patS5 to patS8 inhibited heterocyst formation but patS4 did not. In contrast to the full-length patS gene, P(hepA)-patS5 failed to restore a wild-type pattern in a patS null mutant, indicating that PatS-5 cannot function in cell-to-cell signaling if it is expressed in proheterocysts. To establish the location of the PatS receptor, PatS-5 was confined within the cytoplasm as a gfp-patS5 fusion. The green fluorescent protein GFP-PatS-5 fusion protein inhibited heterocyst formation. Similarly, full-length PatS with a C-terminal hexahistidine tag inhibited heterocyst formation. These data indicate that the PatS receptor is located in the cytoplasm, which is consistent with recently published data indicating that HetR is a PatS target. We speculated that overexpression of other Anabaena strain PCC 7120 RGSGR-encoding genes might show heterocyst inhibition activity. In addition to patS and hetN, open reading frame (ORF) all3290 and an unannotated ORF, orf77, encode an RGSGR motif. Overexpression of all3290 and orf77 under the control of the petE promoter inhibited heterocyst formation, indicating that the RGSGR motif can inhibit heterocyst development in a variety of contexts.
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