ArticleClinical chemistry2004
Mammaglobin as a novel breast cancer biomarker: multigene reverse transcription-PCR assay and sandwich ELISA.
Article in Clinical chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 60 citations in OpenAlex.
- The Enigma of Mammaglobin: Redefining the Biomarker Paradigm in Breast Carcinoma.International journal of molecular sciences · 2023Review
- Evaluation of Serum Mammaglobin as an Alternative Biomarker in the Diagnosis of Breast Tumors.Journal of laboratory physicians · 2023Article
- Recent advances in biosensing approaches for point-of-care breast cancer diagnostics: challenges and future prospects.Nanoscale advances · 2021Review
- Review
- Detection of human mammaglobin mRNA in breast cancer cells among Vietnamese women.Breast cancer (Dove Medical Press) · 2019Article
- Prognostic role of plasma mammaglobin A expression in breast carcinoma patients: a meta-analysis.OncoTargets and therapy · 2018Review
- Mammaglobin peptide as a novel biomarker for breast cancer detection.Cancer biology & therapy · 2013Article
- Colorimetric immunoassay for detection of tumor markers.International journal of molecular sciences · 2010Article
- Detection of mammaglobin mRNA in peripheral blood is associated with high grade breast cancer: interim results of a prospective cohort study.BMC cancer · 2008Article
- Real-time RT-PCR detection of disseminated tumour cells in bone marrow has superior prognostic significance in comparison with circulating tumour cells in patients with breast cancer.British journal of cancer · 2006Article
- Detection of Survivin-expressing circulating cancer cells in the peripheral blood of breast cancer patients by a RT-PCR ELISA.Clinical & experimental metastasis · 2006Article
- Detection of circulating tumor cells in peripheral blood of breast cancer patients during or after therapy using a multigene real-time RT-PCR assay.Molecular diagnosis & therapy · 2006Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe aim of this study was to examine the potential usefulness of a mammaglobin multigene reverse transcription-PCR (RT-PCR) assay and a mammaglobin sandwich ELISA as diagnostic tools in breast cancer.
methodsWe studied peripheral blood samples from 147 untreated Senegalese women with biopsy-confirmed breast cancer and gathered patient information regarding demographic, and clinical staging of disease. The samples were tested for mammaglobin and three breast cancer-associated gene transcripts by a multigene real-time RT-PCR assay and for serum mammaglobin protein by a sandwich ELISA assay.
resultsIn 77% of the breast cancer blood samples, a positive signal was obtained in the multigene RT-PCR assay detecting mammaglobin and three complementary transcribed genes. Fifty samples from healthy female donors tested negative. Significant correlations were found between mammaglobin protein in serum, presence of mammaglobin mRNA-expressing cells in blood, stage of disease, and tumor size. Circulating mammaglobin protein was detected in 68% of the breast cancer sera, and was increased in 38% in comparison with a mixed control population. The RT-PCR assay and the ELISA for mammaglobin produced a combined sensitivity of 84% and specificity of 97%.
conclusionThe ELISA and RT-PCR for mammaglobin and mammaglobin-producing cells could be valuable tools for diagnosis and prognosis of breast cancer.
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