ArticleThe EMBO journal1992
Colicin A unfolds during its translocation in Escherichia coli cells and spans the whole cell envelope when its pore has formed.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 116 citations in OpenAlex.
- Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity.Journal of structural biology: X · 2026Article
- Colicin E1 opens its hinge to plug TolC.eLife · 2022Article
- Porin threading drives receptor disengagement and establishes active colicin transport through Escherichia coli OmpF.The EMBO journal · 2021Article
- Similarities and Differences between Colicin and Filamentous Phage Uptake by Bacterial Cells.EcoSal Plus · 2019Review
- Genome-Wide Screening Identifies Six Genes That Are Associated with Susceptibility to Escherichia coli Microcin PDI.Applied and environmental microbiology · 2015Article
- Conditionally and transiently disordered proteins: awakening cryptic disorder to regulate protein function.Chemical reviews · 2014Review
- In vivo processing of DNase colicins E2 and E7 is required for their import into the cytoplasm of target cells.PloS one · 2014Article
- Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering.The Journal of biological chemistry · 2012Article
- Swimming against the tide: progress and challenges in our understanding of colicin translocation.Nature reviews. Microbiology · 2010Review
- Fluoresceination of FepA during colicin B killing: effects of temperature, toxin and TonB.Molecular microbiology · 2009Article
- Periplasmic chaperone FkpA is essential for imported colicin M toxicity.Molecular microbiology · 2008Article
- Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavage.Journal of bacteriology · 2008Article
- Colicin N binds to the periphery of its receptor and translocator, outer membrane protein F.Structure (London, England : 1993) · 2008Article
- Colicin E2 is still in contact with its receptor and import machinery when its nuclease domain enters the cytoplasm.Journal of bacteriology · 2007Article
- Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import.The EMBO journal · 2007Article
- Colicin biology.Microbiology and molecular biology reviews : MMBR · 2007Review
- Rapid detection of colicin E9-induced DNA damage using Escherichia coli cells carrying SOS promoter-lux fusions.Journal of bacteriology · 2005Article
- Molecular basis of bacterial outer membrane permeability revisited.Microbiology and molecular biology reviews : MMBR · 2003Review
- Protein translocation across planar bilayers by the colicin Ia channel-forming domain: where will it end?The Journal of general physiology · 2000Article
- Colicin import into Escherichia coli cells.Journal of bacteriology · 1998Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The addition of the pore forming colicin A to Escherichia coli cells results in an efflux of cytoplasmic potassium. This efflux is preceded by a lag time which is related to the time needed for the translocation of the toxin through the envelope. Denaturing the colicin A with urea, before adding it to the cells, did not affect the properties of the pore but decreased the lag time. After renaturation, the lag time was similar to that of the native colicin. This suggests that the unfolding of colicin A accelerates its translocation. The addition of trypsin, which has access neither to the periplasmic space nor to the cytoplasmic membrane, resulted in an immediate arrest of the potassium efflux induced by colicins A and B. The possibility that trypsin may act on a bacterial component required for colicin reception and/or translocation was ruled out. It is thus likely that the arrest of the efflux corresponds to a closing of the pores. This long distance effect of trypsin suggests that part of the polypeptide chain of the colicins may still be in contact with the external medium even when the pore has formed in the inner membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.