ArticleFunctional & integrative genomics2004
Comprehensive metabolite profiling of Sinorhizobium meliloti using gas chromatography-mass spectrometry.
Article in Functional & integrative genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 64 citations in OpenAlex.
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- Protection of Pepper Plants from Drought byFrontiers in microbiology · 2018Article
- A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti.Scientific reports · 2016Article
- A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum.BMC genomics · 2013Article
- Spectral deconvolution for gas chromatography mass spectrometry-based metabolomics: current status and future perspectives.Computational and structural biotechnology journal · 2013Review
- The perinatal transition of the circulating metabolome in a nonhuman primate.Pediatric research · 2012Article
- In silico insights into the symbiotic nitrogen fixation in Sinorhizobium meliloti via metabolic reconstruction.PloS one · 2012Article
- Combinatorial signal integration by APETALA2/Ethylene Response Factor (ERF)-transcription factors and the involvement of AP2-2 in starvation response.International journal of molecular sciences · 2012Article
- Metabolic regulation of trisporic acid on Blakeslea trispora revealed by a GC-MS-based metabolomic approach.PloS one · 2012Article
- The interplay of proton, electron, and metabolite supply for photosynthetic H2 production in Chlamydomonas reinhardtii.The Journal of biological chemistry · 2010Article
- Extraction of pure components from overlapped signals in gas chromatography-mass spectrometry (GC-MS).BioData mining · 2009Article
- Comparative metabolomic analysis of Sinorhizobium sp. C4 during the degradation of phenanthrene.Applied microbiology and biotechnology · 2008Article
- Genomes of the symbiotic nitrogen-fixing bacteria of legumes.Plant physiology · 2007Review
- The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- An integrated approach to functional genomics: construction of a novel reporter gene fusion library for Sinorhizobium meliloti.Applied and environmental microbiology · 2006Article
- Construction of a large signature-tagged mini-Tn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti.Applied and environmental microbiology · 2006Article
- Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A metabolite analysis of the soil bacterium Sinorhizobium meliloti was established as a first step towards a better understanding of the symbiosis with its host plant Medicago truncatula. A crucial step was the development of fast harvesting and extraction methods for the bacterial metabolites because of rapid changes in their composition. S. meliloti 1021 cell cultures grown in minimal medium were harvested by centrifugation, filtration or immediate freezing in liquid nitrogen followed by a lyophilisation step. Bacteria were lysed mechanically in methanol and hydrophilic compounds were analysed after methoxymation and silylisation via GC-MS. The different compounds were identified by comparison with the NIST 98 database and available standards. From about 200 peaks in each chromatogram 65 compounds have been identified so far. A comparison of the different extraction methods giving the metabolite composition revealed clear changes in several amino acids and amino acid precursor pools. A principal component analysis (PCA) was able to distinguish S. meliloti cells grown on different carbon sources based on their metabolite profile. A comparison of the metabolite composition of a S. meliloti leucine auxotrophic mutant with the wild type revealed a marked accumulation of 2-isopropylmalate in the mutant. Interestingly, the accumulated metabolite is not the direct substrate of the mutated enzyme, 3-isopropylmalate dehydrogenase, but the substrate of isopropylmalate isomerase, which acts one step further upstream in the biosynthetic pathway of leucine. This finding further emphasises the importance of integrating metabolic data into post-genomic research.
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