ArticleThe EMBO journal2004
Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 137 citations in OpenAlex.
- Article
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.Journal of cell science · 2024Article
- Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024Review
- Activation of the cell wall integrity pathway negatively regulates TORC2-Ypk1/2 signaling through blocking eisosome disassembly in Saccharomyces cerevisiae.Communications biology · 2024Article
- Casein kinase 1 controls components of a TORC2 signaling network in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Quantifying yeast lipidomics by high-performance thin-layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics.Microbial cell (Graz, Austria) · 2024Article
- The phosphatase Glc7 controls the eisosomal response to starvation via post-translational modification of Pil1.Journal of cell science · 2023Article
- TOR complex 2 is a master regulator of plasma membrane homeostasis.The Biochemical journal · 2022Article
- Stage-Specific Genetic Interaction betweenInternational journal of molecular sciences · 2022Article
- Novel factors contributing to fungal pathogenicity at early stages of Setosphaeria turcica infection.Molecular plant pathology · 2022Article
- A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast.Journal of cell science · 2021Article
- Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole.Biomolecules · 2020Article
- Disruption of Membrane Integrity by the Bacterium-Derived Antifungal Jagaricin.Antimicrobial agents and chemotherapy · 2019Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- Analysis of the roles of phosphatidylinositol-4,5-Molecular biology of the cell · 2019Article
- Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2.Genes & development · 2018Article
- Heterologous mammalian Akt disrupts plasma membrane homeostasis by taking over TORC2 signaling in Saccharomyces cerevisiae.Scientific reports · 2018Article
- Cell Size and Growth Rate Are Modulated by TORC2-Dependent Signals.Current biology : CB · 2018Article
- The TORC2-Dependent Signaling Network in the Yeast Saccharomyces cerevisiae.Biomolecules · 2017Review
- Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.The Journal of cell biology · 2017Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
To further understand the roles played by the essential phosphoinositide PI4,5P(2), we have used a synthetic lethal analysis, which systematically combined the mss4(ts) mutation, partially defective in PI4P 5-kinase activity, with each of approximately 4700 deletion mutations. This genomic screening technique uncovered numerous new candidate effectors and regulators of PI4,5P(2) in yeast. In particular, we identified Slm1 (Yil105c), a previously uncharacterized PI4,5P(2) binding protein. Like Mss4, Slm1 and its homolog Slm2 (Ynl047c) were required for actin cytoskeleton polarization and viability. Co-immunoprecipitation experiments revealed that Slm1 interacts with a component of TORC2, a Tor2 kinase-containing complex, which also regulates the actin cytoskeleton. Consistent with these findings, phosphorylation of Slm1 and Slm2 was dependent on TORC2 protein kinase activity, both in vivo and in vitro, and Slm1 localization required both PI4,5P(2) and functional TORC2. Together, these data suggest that Slm1 and Slm2 function downstream of PI4,5P(2) and the TORC2 kinase pathway to control actin cytoskeleton organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.