ArticleGenes & development2004
A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 252 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
252 citing papers in PubMed, 410 citations in OpenAlex.
- Dual Roads of CENP-A for Centromere Identification.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Article
- Sustainable integrative cell biology: CENP-C is guilty by association.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Genetic and physical interactions reveal overlapping and distinct contributions to meiotic double-strand break formation inbioRxiv : the preprint server for biology · 2025Article
- Article
- The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms.Communications biology · 2025Article
- Review
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells.PLoS genetics · 2024Article
- Regulation of outer kinetochore assembly during meiosis I and II by CENP-A and KNL-2/M18BP1 in C. elegans oocytes.Current biology : CB · 2024Article
- Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore.Nature communications · 2024Article
- Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes.Nature communications · 2023Article
- Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution.The Journal of cell biology · 2023Article
- The Loss-Function ofSensors (Basel, Switzerland) · 2023Article
- Article
- Role of Kinetochore Scaffold 1 (KNL1) in Tumorigenesis and Tumor Immune Microenvironment in Pan-Cancer: Bioinformatics Analyses and Validation of Expression.International journal of general medicine · 2023Article
- Cell cycle control of kinetochore assembly.Nucleus (Austin, Tex.) · 2022Review
- MEL-28/ELYS and CENP-C coordinately control outer kinetochore assembly and meiotic chromosome-microtubule interactions.Current biology : CB · 2022Article
- Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.The Journal of cell biology · 2022Article
- The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.Frontiers in cell and developmental biology · 2022Review
192 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from Caenorhabditis elegans, seven of which were previously uncharacterized. Using in vivo assays to monitor chromosome segregation, kinetochore assembly, and the mechanical stability of chromosome-microtubule attachments, we show that this copurifying protein network plays a central role at the kinetochore-microtubule interface. In addition, our analysis suggests that the network is comprised of three groups of proteins that contribute in distinct ways to this interface: KNL proteins act after the assembly of centromeric chromatin to generate the core of the microtubule-binding interface, MIS proteins control the rate and extent of formation of this interface, and NDC proteins are necessary to sustain tension during interactions with spindle microtubules. We also purify a similar set of associated proteins from human cells that includes four novel proteins and has recognizable homologs from each functional class. Thus, this protein network is a conserved constituent of the outer kinetochore, and the functions defined by our analysis in C. elegans are likely to be widely relevant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.