ArticleGenes & development2004
A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 142 citations in OpenAlex.
- Review
- Separable roles for RNAi in regulation of transposable elements and viability in the fission yeast Schizosaccharomyces japonicus.PLoS genetics · 2022Article
- Article
- An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing.PLoS genetics · 2021Article
- A Glimpse of "Dicer Biology" Through the Structural and Functional Perspective.Frontiers in molecular biosciences · 2021Review
- Article
- Expression ofGenes · 2020Article
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
- AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.Frontiers in bioscience (Landmark edition) · 2020Review
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- Functional lability of RNA-dependent RNA polymerases in animals.PLoS genetics · 2019Article
- Programmable RNA recognition using a CRISPR-associated Argonaute.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.Molecular cell · 2017Article
- Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.Molecular biology of the cell · 2017Article
- The RNA-induced transcriptional silencing complex targets chromatin exclusively via interacting with nascent transcripts.Genes & development · 2016Article
- Two sets of RNAi components are required for heterochromatin formation in trans triggered by truncated transgenes.Nucleic acids research · 2016Article
- The inhibition of microRNAs by HIV-1 Tat suppresses beta catenin activity in astrocytes.Retrovirology · 2016Article
- Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus.World journal of virology · 2015Review
- RNAi and heterochromatin assembly.Cold Spring Harbor perspectives in biology · 2015Review
- Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum.Scientific reports · 2015Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Schizosaccharomyces pombe genome encodes only one of each of the three major classes of proteins implicated in RNA silencing: Dicer (Dcr1), RNA-dependent RNA polymerase (RdRP; Rdp1), and Argonaute (Ago1). These three proteins are required for silencing at centromeres and for the initiation of transcriptionally silent heterochromatin at the mating-type locus. Here, we show that the introduction of a double-stranded RNA (dsRNA) hairpin corresponding to a green fluorescent protein (GFP) transgene triggers classical RNA interference (RNAi) in S. pombe. That is, GFP silencing triggered by dsRNA reflects a change in the steady-state concentration of GFP mRNA, but not in the rate of GFP transcription. RNAi in S. pombe requires dcr1, rdp1, and ago1, but does not require chp1, tas3, or swi6, genes required for transcriptional silencing. Thus, the RNAi machinery in S. pombe can direct both transcriptional and posttranscriptional silencing using a single Dicer, RdRP, and Argonaute protein. Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.