ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed.
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- Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers.Journal, genetic engineering & biotechnology · 2025Article
- Genetic diversity and antifungal resistance in Candida albicans from VVC cases in Indian women.BMC microbiology · 2025Article
- Genome-wide association study as a powerful tool for dissecting competitive traits in legumes.Frontiers in plant science · 2023Review
- Genetics, genomics, and breeding of black gram [Frontiers in plant science · 2023Review
- A CRISPR-based lateral flow assay for plant genotyping and pathogen diagnostics.Plant biotechnology journal · 2022Article
- Long-read-based draft genome sequence of Indian black gram IPU-94-1 'Uttara': Insights into disease resistance and seed storage protein genes.The plant genome · 2022Article
- Molecular characterization and genetic diversity studies of Indian soybean (Glycine max (L.) Merr.) cultivars using SSR markers.Molecular biology reports · 2022Article
- Vignette ofFrontiers in genetics · 2022Review
- Multiple DNA marker-assisted diversity analysis of Indian mango (Mangifera indica L.) populations.Scientific reports · 2021Article
- Exploring the Genetic Divergence in Mungbean (Vigna radiata L.) Germplasm Using Multiple Molecular Marker Systems.Molecular biotechnology · 2020Article
- Genetic structure and diversity analysis of tall fescue populations by EST-SSR and ISSR markers.Molecular biology reports · 2020Article
- Genetic diversity and population structure detection in sponge gourd (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Assessment of genetic diversity and relationships amongJournal of genetics · 2019Article
- Article
- Genetic diversity and structure ofHereditas · 2019Article
- Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers.BMC genetics · 2017Article
- Assessment of genetic diversity and population structure of an endemic Moroccan tree (Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm.3 Biotech · 2017Article
- Genetic diversity ofPeerJ · 2016Article
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Abstract
Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in elite blackgram genotypes. A total of 25 random and 16 ISSR primers were used. Amplification of genomic DNA of the 18 genotypes, using RAPD analysis, yielded 104 fragments that could be scored, of which 44 were polymorphic, with an average of 1.8 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from two (OPA-13) to nine (OPK-4) and varied in size from 200 bp to 2,500 bp. Percentage polymorphism ranged from 16.6% (OPK-7) to a maximum of 66.6% (OPE-5, OPH-2, and OPK-8), with an average of 42.7%. The 16 ISSR primers used in the study produced 101 bands across 18 genotypes, of which 55 were polymorphic. The number of amplified bands varied from two (ISSR 858) to ten (ISSR 810), with a size range of 200-2,200 bp. The average numbers of bands per primer and polymorphic bands per primer were 6.3 and 3.4, respectively. Percentage polymorphism ranged from 25% (ISSR 885) to 100% (ISSR 858), with an average percentage polymorphism of 57.5% across all the genotypes. The 3'-anchored primers based on poly(GA) and poly(AG) motifs produced high average polymorphisms of 54.98% and 58.32%, respectively. ISSR markers were more efficient than the RAPD assay, as they detected 57.4% polymorphic DNA markers in Vigna mungo as compared to 42.7% for RAPD markers. The Mantel test between the two Jaccard's similarity matrices gave r=0.32, showing low correlation between RAPD- and ISSR-based similarities. Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrogram were compared, whereas the pattern of clustering of the genotypes remained more or less the same in ISSR and combined data of RAPD and ISSR.
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