ArticleIntensive care medicine2004
Immune monitoring of patients with septic shock by measurement of intraleukocyte cytokines.
Article in Intensive care medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- Triggering receptor expressed on myeloid cells-1 (Trem-1) on blood neutrophils is associated with cytokine inducibility in human E. coli sepsis.Diagnostic pathology · 2013Article
- Severity of the compensatory anti-inflammatory response determined by monocyte HLA-DR expression may assist outcome prediction in cirrhosis.Intensive care medicine · 2011Article
- Differential down-regulation of HLA-DR on monocyte subpopulations during systemic inflammation.Critical care (London, England) · 2010Article
- Cecal ligation and puncture induced sepsis impairs host defense against Enterococcus faecium peritonitis.Intensive care medicine · 2009Article
- Phenotype changes and impaired function of dendritic cell subsets in patients with sepsis: a prospective observational analysis.Critical care (London, England) · 2009Article
- "Host tissue damage" signal ATP impairs IL-12 and IFNgamma secretion in LPS stimulated whole human blood.Intensive care medicine · 2008Article
- Elevation in interleukin 13 levels in patients diagnosed with systemic inflammatory response syndrome.Intensive care medicine · 2006Article
- A predominantly anti-inflammatory cytokine profile is associated with disease severity in meningococcal sepsis.Intensive care medicine · 2005Article
- The selective alpha7 agonist GTS-21 attenuates cytokine production in human whole blood and human monocytes activated by ligands for TLR2, TLR3, TLR4, TLR9, and RAGE.Molecular medicine (Cambridge, Mass.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo assess the immune competence of patients presenting with septic shock by measuring on-line the production of intracellular cytokines by circulating leukocytes. DESIGN AND
settingProspective study in a 18-bed medical intensive care unit of a university hospital. PATIENTS AND
participants21 patients with septic shock, and 11 volunteers.
interventionsSingle-step isolation of leukocytes from whole blood obtained within the first 24 h after admission. Leukocytes were fixed immediately or after treatment with lipopolysaccharide (LPS) and/or heterologous plasma. MEASUREMENTS AND
resultsLeukocytes were permeabilized, and the intracellular cytokine expression of TNF-alpha and IL-10 was quantified by immunostaining and flow cytometry. LPS treatment significantly increased monocyte intracellular cytokine TNF-alpha and IL-10 as well as lymphocyte intracellular cytokine IL-10 in normal leukocytes. Septic monocytes and granulocytes had nonstimulated intracellular cytokine TNF-alpha concentrations lower than those measured in volunteers and were severely hyporesponsive to LPS. These phenotypic changes were correlated with disease severity and could be reproduced by treatment of normal leukocytes with plasma from patients with septic shock.
conclusionsIntracellular cytokine staining is a simple and rapid method to assess in situ and on-line the inflammatory balance and responsiveness of leukocyte subpopulations and could therefore represent a useful monitoring tool to assess the immune competence of critically ill patients. This study identifies the cellular source of cytokines in whole blood and confirms prior reports showing that septic phagocytes are characterized by a predominant anti-inflammatory phenotype, with hyporesponsiveness to LPS, depending on a plasma deactivation factor.
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Registered trials
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