ArticleThe Plant cell2004
Silencing in Arabidopsis T-DNA transformants: the predominant role of a gene-specific RNA sensing mechanism versus position effects.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed, 283 citations in OpenAlex.
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
- Efficient site-specific gene addition using R2 retrotransposons in tobacco and rice.Nature biotechnology · 2026Article
- Distinct thresholds condition sense posttranscriptional gene silencing initiation and amplification.The Plant cell · 2026Article
- Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing.Plant physiology · 2026Article
- EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants.Plant biotechnology journal · 2026Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Epigenetic Regulation of Salt Stress Responses in Rice: Mechanisms and Prospects for Enhancing Tolerance.Epigenomes · 2025Review
- Aberrant RNA identification reveals triggers of transgene silencing.The Plant cell · 2025Article
- The genetic basis of replicated bullseye pattern reduction across the Hibiscus trionum complex.The New phytologist · 2025Article
- SeedSeg: image-based transgenic seed counting for segregation analysis of T-DNA loci.Plant methods · 2025Article
- Suppression of plastid-to-nucleus gene transfer by DNA double-strand break repair.Nature plants · 2025Article
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- Interplay between Agrobacterium T-DNA and backbone DNA in transgenic plant cells.Transgenic research · 2024Article
- Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits.The Plant cell · 2024Article
- BdRCN4, a Brachypodium distachyon TFL1 homologue, is involved in regulation of apical meristem fate.Plant molecular biology · 2024Article
- Functional evidence on the involvement of theFrontiers in plant science · 2024Article
- A cell-based fluorescent system and statistical framework to detect meiosis-like induction in plants.Frontiers in plant science · 2024Article
- Evidence of a novel silencing effect on transgenes in the Arabidopsis thaliana sperm cell.The Plant cell · 2023Article
- Floral Development onPlants (Basel, Switzerland) · 2023Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pronounced variability of transgene expression and transgene silencing are commonly observed among independent plant lines transformed with the same construct. Single-copy T-DNA lines harboring reporter genes of various kind and number under the control of a strong promoter were established in Arabidopsis thaliana for a comprehensive analysis of transgene expression. Characterization of 132 independent transgenic lines revealed no case of silencing as a result of site of T-DNA integration. Below a certain number of identical transgenes in the genome, gene copy number and expression were positively correlated. Expression was high, stable over all generations analyzed, and of a comparable level among independent lines harboring the same copy number of a particular transgene. Conversely, RNA silencing was triggered if the transcript level of a transgene surpassed a gene-specific threshold. Transcript level-mediated silencing effectively accounts for the pronounced transgene expression variability seen among transformants. It is proposed that the RNA sensing mechanism described is a genome surveillance system that eliminates RNA corresponding to excessively transcribed genes, including transgenes, and so plays an important role in genome defense.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.