ArticleNucleic acids research2004
Determination and augmentation of RNA sequence specificity of the Nova K-homology domains.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- K-Homology Splicing Regulatory Protein (KSRP) Augments Survival and Proliferation of Human Melanoma Cells.Current issues in molecular biology · 2025Article
- K-Homology Type Splicing Regulatory Protein: Mechanism of Action in Cancer and Immune Disorders.Critical reviews in eukaryotic gene expression · 2024Article
- Evidence Favoring a Positive Feedback Loop for Physiologic Auto Upregulation of hnRNP-E1 during Prolonged Folate Deficiency in Human Placental Cells.The Journal of nutrition · 2017Article
- Review
- TOX4 and NOVA1 proteins are partners of the LEDGF PWWP domain and affect HIV-1 replication.PloS one · 2013Article
- Combinatorial mutagenesis of MBNL1 zinc fingers elucidates distinct classes of regulatory events.Molecular and cellular biology · 2012Article
- Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions.Genome biology · 2012Article
- Protein-RNA and protein-protein recognition by dual KH1/2 domains of the neuronal splicing factor Nova-1.Structure (London, England : 1993) · 2011Article
- The roles of S1 RNA-binding domains in Rrp5's interactions with pre-rRNA.RNA (New York, N.Y.) · 2011Article
- Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast.RNA (New York, N.Y.) · 2008Article
- Towards deciphering the principles underlying an mRNA recognition code.Current opinion in structural biology · 2008Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Nova onconeural antigens are implicated in the pathogenesis of paraneoplastic opsoclonus-myoclonus-ataxia (POMA). The Nova antigens are neuron-specific RNA-binding proteins harboring three repeats of the K-homology (KH) motif; they have been implicated in the regulation of alternative splicing of a host of genes involved in inhibitory synaptic transmission. Although the third Nova KH domain (KH3) has been extensively characterized using biochemical and crystallographic techniques, the roles of the KH1 and KH2 domains remain unclear. Furthermore, the specificity determinants that distinguish the Nova KH domains from those of the closely related hnRNP E and hnRNP K proteins are undefined. We demonstrate through the use of RNA selection and biochemical analysis that the sequence specificity of the Nova KH1/2 domains is similar to that of Nova KH3. We also show that the mutagenesis of a Nova KH domain to render it similar to the KH domains of the heterogeneous nuclear ribonucleoprotein E (hnRNP E) and hnRNP K allow it to recognize longer RNA sequences. These data yield important insights into KH domain function and suggest a strategy by which to engineer KH domains with novel sequence preferences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.